• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

核蛋白颗粒的HMG 14/17结合亲和力及DNA酶I敏感性

HMG 14/17 binding affinities and DNAase I sensitivities of nucleoprotein particles.

作者信息

Stein A, Townsend T

出版信息

Nucleic Acids Res. 1983 Oct 11;11(19):6803-19. doi: 10.1093/nar/11.19.6803.

DOI:10.1093/nar/11.19.6803
PMID:6226937
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326415/
Abstract

We show that ordinary (bulk) chicken erythrocyte nucleosomes are digested more rapidly by DNAase I when they are associated with high mobility group (HMG) proteins 14/17. Digestion of HMG 14/17-nucleosome complexes, under conditions where the DNA in control nucleosomes is digested to 10 to 20% acid solubility, results in a particular depletion of single-strand DNA fragments greater than 80 nucleotides in length, relative to the DNA fragments produced from control nucleosomes. Additionally, we show that staphylococcal nuclease digests of H1/H5-depleted chromatin contain an abundant subclass of nucleosomes that are not present in appreciable amounts in digests of native chromatin. These nucleosomes contain longer lengths of DNA and have lower electrophoretic mobilities than core particles. HMG 14/17 associates highly preferentially with these nucleosomes and renders them sensitive to DNAase I, similar to what has been found for active nucleosomes.

摘要

我们发现，普通（整体）鸡红细胞核小体与高迁移率族（HMG）蛋白14/17结合时，被DNA酶I消化得更快。在对照核小体中的DNA被消化至酸溶性为10%至20%的条件下，HMG 14/17-核小体复合物的消化导致长度大于80个核苷酸的单链DNA片段相对于对照核小体产生的DNA片段有特定程度的减少。此外，我们表明，H1/H5缺失染色质经葡萄球菌核酸酶消化后，含有一类丰富的核小体亚类，而天然染色质消化产物中不存在大量此类核小体。这些核小体含有更长的DNA片段，并且电泳迁移率比核心颗粒低。HMG 14/17高度优先地与这些核小体结合，并使其对DNA酶I敏感，这与活性核小体的情况类似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9cb/326415/07e8e5da5153/nar00364-0232-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9cb/326415/d727636c46f4/nar00364-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9cb/326415/f41c2acbee96/nar00364-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9cb/326415/5cf17a914a9d/nar00364-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9cb/326415/07e8e5da5153/nar00364-0232-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9cb/326415/d727636c46f4/nar00364-0227-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9cb/326415/f41c2acbee96/nar00364-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9cb/326415/5cf17a914a9d/nar00364-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e9cb/326415/07e8e5da5153/nar00364-0232-a.jpg

相似文献

1
HMG 14/17 binding affinities and DNAase I sensitivities of nucleoprotein particles.核蛋白颗粒的HMG 14/17结合亲和力及DNA酶I敏感性
Nucleic Acids Res. 1983 Oct 11;11(19):6803-19. doi: 10.1093/nar/11.19.6803.
2
Isolation of high mobility group-containing mononucleosomes from avian erythrocyte nuclei and their sensitivity to DNase I.从禽红细胞核中分离含高迁移率族的单核小体及其对DNase I的敏感性
J Biol Chem. 1982 Nov 10;257(21):13088-94.
3
The interaction of high mobility proteins HMG14 and 17 with nucleosomes.高迁移率蛋白HMG14和17与核小体的相互作用。
Nucleic Acids Res. 1980 Sep 11;8(17):3757-78. doi: 10.1093/nar/8.17.3757.
4
Influence of nonhistone chromatin protein HMG-1 on the enzymatic digestion of purified DNA.非组蛋白染色质蛋白HMG-1对纯化DNA酶促消化的影响。
Nucleic Acids Res. 1982 Aug 25;10(16):5059-72. doi: 10.1093/nar/10.16.5059.
5
Electrophoretic separation of a class of nucleosomes enriched in HMG 14 and 17 and actively transcribed globin genes.一类富含高迁移率族蛋白14和17以及活跃转录的珠蛋白基因的核小体的电泳分离。
Nucleic Acids Res. 1980 Jun 25;8(12):2787-805. doi: 10.1093/nar/8.12.2787.
6
Preferential in vitro binding of high mobility group proteins 14 and 17 to nucleosomes containing active and DNase I sensitive single-copy genes.高迁移率族蛋白14和17在体外优先结合含有活性和对脱氧核糖核酸酶I敏感的单拷贝基因的核小体。
Biochemistry. 1986 Jun 3;25(11):3447-54. doi: 10.1021/bi00359a053.
7
The footprint of chromosomal proteins HMG-14 and HMG-17 on chromatin subunits.染色体蛋白HMG-14和HMG-17在染色质亚基上的印记。
J Mol Biol. 1994 Feb 11;236(1):189-98. doi: 10.1006/jmbi.1994.1128.
8
Nonhistone nuclear high mobility group proteins 14 and 17 stabilize nucleosome core particles.非组蛋白核高迁移率族蛋白14和17可稳定核小体核心颗粒。
J Biol Chem. 1983 Nov 10;258(21):13221-9.
9
Neutron scattering studies and modeling of high mobility group 14 core nucleosome complex.高迁移率族14核心核小体复合物的中子散射研究与建模
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5258-62. doi: 10.1073/pnas.79.17.5258.
10
Isolation of actively transcribed nucleosomes using immobilized HMG 14 and 17 and an analysis of alpha-globin chromatin.使用固定化的HMG 14和17分离活跃转录的核小体并分析α-珠蛋白染色质。
Cell. 1981 Feb;23(2):391-400. doi: 10.1016/0092-8674(81)90134-3.

引用本文的文献

1
Transcriptional regulation by HMGN proteins.HMGN蛋白的转录调控
Biochim Biophys Acta. 2010 Jan-Feb;1799(1-2):74-9. doi: 10.1016/j.bbagrm.2009.11.006.
2
Neither HMG-14a nor HMG-17 gene function is required for growth of chicken DT40 cells or maintenance of DNaseI-hypersensitive sites.鸡DT40细胞的生长或DNaseI超敏位点的维持均不需要HMG - 14a和HMG - 17基因的功能。
Nucleic Acids Res. 1997 Jan 15;25(2):283-8. doi: 10.1093/nar/25.2.283.
3
Interaction between domains in chromosomal protein HMG-1.染色体蛋白HMG-1中各结构域之间的相互作用。

本文引用的文献

1
THE MELTING TRANSITION OF LOW-MOLECULAR-WEIGHT DNA: THEORY AND EXPERIMENT.低分子量DNA的解链转变：理论与实验
J Mol Biol. 1965 Apr;11:802-20. doi: 10.1016/s0022-2836(65)80037-7.
2
Nucleosome cores have two specific binding sites for nonhistone chromosomal proteins HMG 14 and HMG 17.核小体核心对非组蛋白染色体蛋白HMG 14和HMG 17有两个特定的结合位点。
Science. 1980 Sep 26;209(4464):1534-6. doi: 10.1126/science.7433974.
3
Subunit structures of different electrophoretic forms of nucleosomes.核小体不同电泳形式的亚基结构。
EMBO J. 1984 Jun;3(6):1255-61. doi: 10.1002/j.1460-2075.1984.tb01960.x.
4
Chromatin from transcribed genes contains HMG17 only downstream from the starting point of transcription.来自转录基因的染色质仅在转录起始点下游含有高迁移率族蛋白17（HMG17）。
EMBO J. 1987 Aug;6(8):2393-9. doi: 10.1002/j.1460-2075.1987.tb02517.x.
5
The structure of nucleosomal core particles within transcribed and repressed gene regions.转录和抑制基因区域内核小体核心颗粒的结构。
Nucleic Acids Res. 1988 Dec 9;16(23):11187-205. doi: 10.1093/nar/16.23.11187.
6
Recombinant human chromosomal proteins HMG-14 and HMG-17.重组人染色体蛋白HMG - 14和HMG - 17
Nucleic Acids Res. 1991 Jun 11;19(11):3115-21. doi: 10.1093/nar/19.11.3115.
7
Heparin increases chromatin accessibility by binding the trypsin-sensitive basic residues in histones.肝素通过结合组蛋白中对胰蛋白酶敏感的碱性残基来增加染色质的可及性。
Biochem J. 1992 Dec 15;288 ( Pt 3)(Pt 3):953-8. doi: 10.1042/bj2880953.
J Biol Chem. 1980 Apr 25;255(8):3673-84.
4
Histone H5 can increase the internucleosome spacing in dinucleosomes to nativelike values.组蛋白H5可将双核小体中的核小体间距增加至天然状态的值。
Biochemistry. 1983 Apr 12;22(8):1783-9. doi: 10.1021/bi00277a007.
5
Histone H5 can correctly align randomly arranged nucleosomes in a defined in vitro system.组蛋白H5能够在特定的体外系统中使随机排列的核小体正确排列。
Nature. 1983 Apr 7;302(5908):548-50. doi: 10.1038/302548a0.
6
The interaction of high mobility proteins HMG14 and 17 with nucleosomes.高迁移率蛋白HMG14和17与核小体的相互作用。
Nucleic Acids Res. 1980 Sep 11;8(17):3757-78. doi: 10.1093/nar/8.17.3757.
7
Electrophoretic separation of a class of nucleosomes enriched in HMG 14 and 17 and actively transcribed globin genes.一类富含高迁移率族蛋白14和17以及活跃转录的珠蛋白基因的核小体的电泳分离。
Nucleic Acids Res. 1980 Jun 25;8(12):2787-805. doi: 10.1093/nar/8.12.2787.
8
Chromatin structure of the ovalbumin gene family in the chicken oviduct.鸡输卵管中卵清蛋白基因家族的染色质结构。
Biochemistry. 1983 Jan 4;22(1):21-30. doi: 10.1021/bi00270a004.
9
Affinity of HMG17 for a mononucleosome is not influenced by the presence of ubiquitin-H2A semihistone but strongly depends on DNA fragment size.HMG17对单核小体的亲和力不受泛素-H2A半组蛋白存在的影响，但强烈依赖于DNA片段大小。
Nucleic Acids Res. 1983 Jan 25;11(2):387-401. doi: 10.1093/nar/11.2.387.
10
Interaction of HMG 14 and 17 with actively transcribed genes.HMG 14和17与活跃转录基因的相互作用。
Cell. 1980 Jan;19(1):289-301. doi: 10.1016/0092-8674(80)90410-9.