Chueh L L, Chu R M, Li W I, Chang W F
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1982 Aug;15(3):212-20.
Large numbers of viral particles resembling rotavirus were detected with negative stained electron microscopy in bacteria free fecal filtrate obtained from 10-day old diarrheal suckling piglets of a conventional pig farm in Taiwan. The clinical signs of vomiting and diarrhea were reproduced in colostrum deprived piglets artificially infected with the fecal filtrates. Rotavirus particles persisted in the fecal samples after two in vivo serial passages, and was not seen in the uninfected control animal. The Cytoplasm of infected jejunal and ileal enterocyte fluoresced when standard anti-porcine rotavirus conjugate was applied in an direct immunofluorescent staining test. In the experimentally infected piglets, moderate villous atrophy of the small intestine was the main microscopic lesion observed. The virus was identified by the above evidence to be rotavirus.
在台湾一个传统养猪场10日龄腹泻哺乳仔猪的无菌粪便滤液中，通过负染色电子显微镜检测到大量类似轮状病毒的病毒颗粒。用粪便滤液人工感染初乳缺乏的仔猪后，出现了呕吐和腹泻的临床症状。粪便样本在两次体内连续传代后仍存在轮状病毒颗粒，而未感染的对照动物中未观察到。在直接免疫荧光染色试验中，当应用标准抗猪轮状病毒结合物时，感染的空肠和回肠肠细胞的细胞质发出荧光。在实验感染的仔猪中，观察到的主要微观病变是小肠中度绒毛萎缩。根据上述证据，该病毒被鉴定为轮状病毒。
