Patel D J, Pardi A, Itakura K
Science. 1982 May 7;216(4546):581-90. doi: 10.1126/science.6280281.
The conformation and dynamics of the d(CGCGAATTCGCG) duplex, its analogs containing mismatched base pairs and helix interruptions, and its complexes with actinomycin and Netropsin, bound separately and simultaneously, have been investigated by nuclear magnetic resonance spectroscopy in aqueous solution. Structural information has been deduced from chemical shift and nuclear Overhauser effect parameters, while the kinetics have been probed from line width and saturation recovery experiments on proton and phosphorus markers at the individual base pair level. These studies lead to an improved understanding of the role of nucleic acid sequence on the structure, flexibility, and conformational interconversions in the duplex state. The nuclear magnetic resonance measurements readily identify helix modification and antibiotic binding sites on the nucleic acid and estimate the extent to which the observed conformational and dynamic perturbations are transmitted to adjacent base pair regions.
通过水溶液中的核磁共振光谱法，研究了d(CGCGAATTCGCG)双链体、其包含错配碱基对和螺旋中断的类似物以及分别和同时与放线菌素和纺锤菌素结合形成的复合物的构象和动力学。从化学位移和核Overhauser效应参数推导出结构信息，而动力学则通过在单个碱基对水平上对质子和磷标记物进行线宽和饱和恢复实验来探究。这些研究有助于更好地理解核酸序列在双链状态下的结构、灵活性和构象互变中的作用。核磁共振测量能够轻松识别核酸上的螺旋修饰和抗生素结合位点，并估计观察到的构象和动态扰动传递到相邻碱基对区域的程度。
