金属硫蛋白的生物学功能。V. 各种应激对大鼠的诱导作用。

Biological function of metallothionein. V. Its induction in rats by various stresses.

作者信息

Oh S H, Deagen J T, Whanger P D, Weswig P H

出版信息

Am J Physiol. 1978 Mar;234(3):E282-5. doi: 10.1152/ajpendo.1978.234.3.E282.

DOI:10.1152/ajpendo.1978.234.3.E282

Abstract

Induction of metallothionein (a Zn-binding protein) was investigated in liver and kidneys of rats under the following stresses: cold environment, hot environment, heat burn, strenuous exercise, and CCl4 intoxication. As markers of metallothionein (MT) synthesis, [14C]cystine and 65Zn were injected parenterally into rats, and MT in liver and kidney supernatants was separated on Sephadex G-75 columns. Among stresses tested, the greatest stimulations of MT synthesis in liver were observed under cold (315%), strenuous exercise (302%), and CCl4 intoxication (543%), as evidenced by increased amount of [14C]cystine and 65Zn incorporation into the MT fraction. In contrast to liver, only small responses were observed in MT synthesis in kidneys. There was a general decline in plasma Zn levels in rats under the stresses employed. However, no great differences were observed in Zn levels in whole liver and kidneys. The present study suggests that various unrelated physiological stresses, which are accompanied with a change in Zn distribution, increase the synthesis of MT in liver of rats.

摘要

在以下应激条件下，研究了大鼠肝脏和肾脏中金属硫蛋白（一种锌结合蛋白）的诱导情况：寒冷环境、炎热环境、热烧伤、剧烈运动和四氯化碳中毒。作为金属硫蛋白（MT）合成的标志物，将[14C]胱氨酸和65Zn经肠外注射到大鼠体内，并在葡聚糖凝胶G - 75柱上分离肝脏和肾脏上清液中的MT。在所测试的应激条件中，在寒冷（315%）、剧烈运动（302%）和四氯化碳中毒（543%）条件下，肝脏中MT合成的刺激作用最为显著，这通过[14C]胱氨酸和65Zn掺入MT组分的量增加得以证明。与肝脏不同，在肾脏中MT合成仅观察到较小的反应。在所采用的应激条件下，大鼠血浆锌水平普遍下降。然而，全肝和肾脏中的锌水平未观察到显著差异。本研究表明，各种不相关的生理应激，伴随着锌分布的变化，会增加大鼠肝脏中MT 的合成。