Kodama H, Sato G, Miura S
Avian Dis. 1975 Jul-Sep;19(3):556-65.
Monolayer cell cultures consisting of epithelioid cells were made from pancreatic tissue of 10-to-13-day-old chicks. The maximum virus titer of the cell-culture fluid was obtained 8 days after inoculation with an embryo-adapted avian encephalomyelitis virus (AEV). Virus titers also increased in cell cultures inoculated with a chick-pancreas-passed AEV or a field isolant. Cell cultures inoculated with 3 strains of AEV maintained virus titers of 10(2.9)-10(3.7) 50% embryo-infective doses/ml for 15-20 days. In other cell cultures from pancreatic tissues of chicks preinfected orally with the chick-pancreas-passed AEV or the field isolant, the virus titers decreased for several days after cultivation and thereafter increased and persisted until at least the 25th or 30th day. Neither a cytopathic effect nor any inclusion body was observed in the cell cultures infected with AEV. No AEV-antigen-positive cell was detected by direct fluorescent-antibody technique.
从10至13日龄雏鸡的胰腺组织制备了由上皮样细胞组成的单层细胞培养物。用胚胎适应的禽脑脊髓炎病毒（AEV）接种后8天获得了细胞培养液的最高病毒滴度。用鸡胰腺传代的AEV或田间分离株接种的细胞培养物中病毒滴度也有所增加。接种3株AEV的细胞培养物在15至20天内维持10(2.9)-10(3.7) 50%胚胎感染剂量/毫升的病毒滴度。在其他用鸡胰腺传代的AEV或田间分离株经口预感染的雏鸡胰腺组织的细胞培养物中，培养后病毒滴度下降了几天，此后升高并持续到至少第25天或第30天。在感染AEV的细胞培养物中未观察到细胞病变效应或任何包涵体。用直接荧光抗体技术未检测到AEV抗原阳性细胞。
