Sklar L A, Finney D A
Cytometry. 1982 Nov;3(3):161-5. doi: 10.1002/cyto.990030304.
The binding of a fluorescent peptide to human neutrophils is analyzed with a fluorescence activated cell sorter. We examine steady-state and kinetic features of the ligand-receptor interaction (in the presence of unbound ligand) and we show tht the number of receptors may be estimated without resorting to any external references for calibration. These methods are applicable to other fluorescent ligands with affinities greater than 10(8) M-1.
使用荧光激活细胞分选仪分析荧光肽与人中性粒细胞的结合。我们研究了配体-受体相互作用的稳态和动力学特征（在存在未结合配体的情况下），并且我们表明无需借助任何外部参考进行校准即可估计受体数量。这些方法适用于亲和力大于10⁸ M⁻¹的其他荧光配体。
