Timmons M S, Bogardus A M, Deonier R C
J Bacteriol. 1983 Jan;153(1):395-407. doi: 10.1128/jb.153.1.395-407.1983.
Three IS5 elements were mapped in overlapping chromosomal segments on a series of F-prime plasmids by restriction analysis and hybridization. IS5A was located clockwise of proA near 6 min, IS5B was located clockwise of purE near 12 min, and IS5C was tentatively located near 14 min on the Escherichia coli K-12 map. The physical structures of nine type II F-prime plasmids that contain chromosomal DNA from this region indicated that these plasmids were excised from the chromosome by recombination between pairs of IS5 elements.
通过限制性分析和杂交，在一系列F-prime质粒的重叠染色体片段中定位了三个IS5元件。IS5A位于proA顺时针方向附近6分钟处，IS5B位于purE顺时针方向附近12分钟处，IS5C暂时位于大肠杆菌K-12图谱上14分钟附近。九个包含该区域染色体DNA的II型F-prime质粒的物理结构表明，这些质粒是通过IS5元件对之间的重组从染色体上切除的。
