Edson C M, Cohen L K, Henle W, Strominger J L
J Immunol. 1983 Feb;130(2):919-24.
Sera from a patient with a chronic Epstein Barr virus (EBV) infection contained unusually high anti-EBV antibody titers (1:2560 to 1:10,240 for EA(D) and 1:5,120 to 1:40,960 for VCA). One of these serum samples was shown by immunoprecipitation to recognize at least 11 EBV-specific proteins from virus producer cells labeled in vivo and 10 EBV-specific proteins from in vitro translations of producer cell mRNA. Six of the in vivo labeled proteins (135,000, 89,000, 50,000 to 55,000 doublet, 46,000, and 34,000 daltons) are "early" by their resistance to phosphonoacetic acid, and five (350,000, 220,000, 160,000, 140,000, and 85,000 daltons) are "late" membrane and capsid proteins. The EBV-specific proteins immunoprecipitated from in vitro translations had molecular masses of 150,000, 140,000, 115,000, 52,000, 50,000, 45,000, 34,000, 29,000, 17,000, and 15,000. Subcellular fractionation studies of cells labeled in vivo revealed that the 135,000-dalton protein and part of the 50,000 to 55,000 dalton protein doublet were found in both the nuclear and the cytoplasmic fractions, and thus are good candidates to be components of the EA(D) diffuse-type immunofluorescence observed with many EA-positive sera.
一名慢性爱泼斯坦-巴尔病毒（EBV）感染患者的血清中，抗EBV抗体滴度异常高（EA(D)为1:2560至1:10240，VCA为1:5120至1:40960）。通过免疫沉淀法显示，其中一份血清样本可识别来自体内标记的病毒产生细胞的至少11种EBV特异性蛋白，以及来自产生细胞mRNA体外翻译产物的10种EBV特异性蛋白。体内标记的6种蛋白（135000、89000、50000至55000双峰、46000和34000道尔顿）因其对膦酰乙酸有抗性而属于“早期”蛋白，5种（350000、220000、160000、140000和85000道尔顿）是“晚期”膜蛋白和衣壳蛋白。从体外翻译产物中免疫沉淀的EBV特异性蛋白的分子量分别为150000、140000、115000、52000、50000、45000、34000、29000、17000和15000。对体内标记细胞的亚细胞分级分离研究表明，135000道尔顿的蛋白以及50000至55000道尔顿双峰蛋白的一部分在细胞核和细胞质分级分离物中均有发现，因此很有可能是许多EA阳性血清中观察到的EA(D)弥漫型免疫荧光的组成成分。
