Nagahama S, Kobatake Y, Kurihara K
J Gen Physiol. 1982 Nov;80(5):785-800. doi: 10.1085/jgp.80.5.785.
The lingual artery of the bullfrog was perfused with artificial solution and the effects of Ca2+, Ca-channel blockers (MnCl2 and verapamil), cGMP, and cAMP added to the perfusing solution of the gustatory nerve responses were examined. The responses to chemical stimuli of group 1 (CaCl2, NaCl, distilled water, D-galactose, and L-threonine) applied to the tongue surface were greatly decreased by a decrease in Ca2+ concentration in the perfusing solution, suppressed by the Ca-channel blockers, enhanced by cGMP, and suppressed by cAMP. The responses to chemical stimuli of group 2 (quinine hydrochloride, theophylline, ethanol, and HCl) were practically not affected by a decrease in Ca2+ concentration, the Ca-channel blockers, cGMP, and cAMP. The responses to the stimuli of group 1 seem to be induced by Ca influx into a taste cell that is triggered by depolarization and modulated by the cyclic nucleotides in a taste cell. The responses to group 2 seem to be induced without accompanying Ca influx.
用人工溶液灌注牛蛙的舌动脉，并检测添加到味觉神经反应灌注溶液中的Ca2+、钙通道阻滞剂（MnCl2和维拉帕米）、cGMP和cAMP的作用。灌注溶液中Ca2+浓度降低会使施加于舌表面的第1组化学刺激（CaCl2、NaCl、蒸馏水、D-半乳糖和L-苏氨酸）的反应大幅降低，钙通道阻滞剂会抑制这些反应，cGMP会增强这些反应，而cAMP会抑制这些反应。第2组化学刺激（盐酸奎宁、茶碱、乙醇和HCl）的反应实际上不受Ca2+浓度降低、钙通道阻滞剂、cGMP和cAMP的影响。对第1组刺激的反应似乎是由Ca2+流入味觉细胞所诱导，这种流入由去极化触发，并由味觉细胞中的环核苷酸调节。对第2组刺激的反应似乎是在没有伴随Ca2+流入的情况下诱导产生的。
