Nairn R S, Adair G M, Humphrey R M
Mol Gen Genet. 1982;187(3):384-90. doi: 10.1007/BF00332616.
Thymidine kinase-deficient Chinese hamster ovary (CHO) cells were genetically transformed with the BamHI restriction fragment encoding the thymidine kinase gene of herpes simplex virus (HSV-tk). We have observed considerable clonal variation among independent CHO sublines with respect to transformation competence for the DNA-mediated gene transfer of HSV-tk. Transformation frequencies greater than or equal to 3 X 10(-4) were observed consistently in one subline, with a transformation efficiency of approximately 1 transformant per ng viral gene. The frequency and efficiency of transformation we observed in this system are at least 10-fold greater than those previously reported for DNA-mediated transformation of CHO cells by HSV-tk. All of the CHO HSV-tk+ transformants examined were stable for the transferred genotype in the absence of selection, and all showed evidence of co-transformation by unselected plasmid pBR322 sequences.
将编码单纯疱疹病毒胸苷激酶基因（HSV - tk）的BamHI限制性片段对胸苷激酶缺陷型中国仓鼠卵巢（CHO）细胞进行基因转化。我们观察到，在独立的CHO亚系中，对于HSV - tk的DNA介导基因转移，转化能力存在相当大的克隆变异。在一个亚系中始终观察到转化频率大于或等于3×10⁻⁴，转化效率约为每纳克病毒基因产生1个转化体。我们在该系统中观察到的转化频率和效率比先前报道的HSV - tk对CHO细胞进行DNA介导转化的频率和效率至少高10倍。在没有选择压力的情况下，所有检测的CHO HSV - tk⁺转化体对于转移的基因型都是稳定的，并且所有转化体都显示出未选择的质粒pBR322序列共转化的证据。
