Melaragno A J, Abdu W A, Katchis R J, Vecchione J J, Valeri C R
Vox Sang. 1982;43(6):321-6. doi: 10.1111/j.1423-0410.1982.tb00030.x.
The equivalent of 5-7 units of platelets, isolated from a single donor with the IBM Blood Cell Processor 2997 using a dual stage separation chamber, was frozen with the cryoprotectant dimethylsulfoxide (DMSO). The DMSO-saline solution was added directly to the platelets, and the platelets were frozen in a polyvinyl chloride plastic bag by storage in a -80 degrees C mechanical freezer. Washing the thawed platelets with a phosphate-buffered sodium chloride-dextrose solution, pH of 5.0, removed about 95% of the DMSO. In vitro freeze-thaw-wash recovery was 80%, and in vivo 51Cr platelet recovery was 31%. Platelet dense body granules were well maintained after freezing, thawing, and washing. This is a safe and effective method of platelet cryopreservation which can be performed in less time than other currently used methods.
使用双级分离腔的IBM血细胞处理器2997从单个供体中分离出相当于5 - 7单位的血小板，并用冷冻保护剂二甲基亚砜（DMSO）冷冻。将DMSO盐水溶液直接加入血小板中，然后将血小板置于聚氯乙烯塑料袋中，储存在-80℃的机械冷冻箱中进行冷冻。用pH值为5.0的磷酸盐缓冲氯化钠 - 葡萄糖溶液洗涤解冻后的血小板，可去除约95%的DMSO。体外冻融洗涤回收率为80%，体内51Cr血小板回收率为31%。血小板致密体颗粒在冷冻、解冻和洗涤后保持良好。这是一种安全有效的血小板冷冻保存方法，与目前使用的其他方法相比，所需时间更短。
