Ishitsuka H, Ninomiya Y T, Ohsawa C, Fujiu M, Suhara Y
Antimicrob Agents Chemother. 1982 Oct;22(4):617-21. doi: 10.1128/AAC.22.4.617.
Studies of various analogs related to the antipicornavirus agent, 4',5-dihydroxy-3,3',7-trimethoxyflavone (Ro 09-0179), led to the identification of 4'-ethoxy-2'-hydroxy-4,6'-dimethoxychalcone (Ro 09-0410), a new and different type of antiviral agent. Ro 09-0410 had a high activity against rhinoviruses but no activity against other picornaviruses. Of 53 rhinovirus serotypes so far tested, 46 were susceptible to Ro 09-0410 in HeLa cell cultures. The concentration of Ro 09-0410 inhibiting 50% of the types of rhinovirus was about 0.03 micrograms/ml, whereas the 50% cytotoxic concentration was 30 microgram/ml. Ro 09-0410 inactivated rhinoviruses in direct dose-, time-, and temperature-dependent fashion. Since infectivity, reduced by exposure to the agent, completely regained the original level by extraction of the agent with chloroform, the inactivation may be associated with the binding of the agent to some specific site of the rhinovirus capsid.
对与抗微小核糖核酸病毒药物4',5 - 二羟基 - 3,3',7 - 三甲氧基黄酮（Ro 09 - 0179）相关的各种类似物进行的研究，促成了一种新型且不同类型的抗病毒药物4'-乙氧基 - 2'-羟基 - 4,6'-二甲氧基查耳酮（Ro 09 - 0410）的鉴定。Ro 09 - 0410对鼻病毒具有高活性，但对其他微小核糖核酸病毒无活性。在迄今测试的53种鼻病毒血清型中，有46种在HeLa细胞培养物中对Ro 09 - 0410敏感。抑制50%类型鼻病毒的Ro 09 - 0410浓度约为0.03微克/毫升，而50%细胞毒性浓度为30微克/毫升。Ro 09 - 0410以直接的剂量、时间和温度依赖性方式使鼻病毒失活。由于通过暴露于该药物而降低的感染性，在用氯仿提取该药物后完全恢复到原始水平，所以这种失活可能与该药物与鼻病毒衣壳的某些特定位点的结合有关。
