Vonderfecht S L, Osburn B I
J Clin Microbiol. 1982 Nov;16(5):935-42. doi: 10.1128/jcm.16.5.935-942.1982.
The local and systemic humoral immune responses to rotavirus were studied in six conventional neonatal calves. Attenuated bovine rotavirus was administered either orally or directly into an isolated intestinal loop. The parameters monitored were neutralizing rotavirus antibody in serum, immunofluorescent and neutralizing rotavirus antibody in intestinal loop washings, and rotavirus antibody-producing cells in intestinal mucosa. An antibody response was observed in the serum and intestinal secretions from one calf only. Viral replication was not detected in the isolated intestinal loop. Rotavirus antibody-producing cells were found in the intestinal mucosa of five calves. Double staining revealed that most of these cells produced antibody of the immunoglobulin A class. The conclusions were: (i) a previously described system to detect rotavirus antibody-producing cells can be used to study immune responses in neonatal calves, (ii) the class or subclass of antibody in rotavirus antibody-producing cells can be determined by double immunofluorescent staining, (iii) neonatal calves respond to rotavirus inoculation with a local immunoglobulin A response, and (iv) most of the rotavirus antibody-producing cells are located in the mucosa of the proximal small intestine.
对六头常规新生犊牛针对轮状病毒的局部和全身体液免疫反应进行了研究。将减毒牛轮状病毒经口或直接注入隔离的肠袢。监测的参数包括血清中的中和轮状病毒抗体、肠袢冲洗液中的免疫荧光和中和轮状病毒抗体，以及肠黏膜中产生轮状病毒抗体的细胞。仅在一头犊牛的血清和肠分泌物中观察到抗体反应。在隔离的肠袢中未检测到病毒复制。在五头犊牛的肠黏膜中发现了产生轮状病毒抗体的细胞。双重染色显示，这些细胞中的大多数产生免疫球蛋白A类抗体。结论如下：(i) 先前描述的检测产生轮状病毒抗体细胞的系统可用于研究新生犊牛的免疫反应；(ii) 产生轮状病毒抗体细胞中的抗体类别或亚类可通过双重免疫荧光染色确定；(iii) 新生犊牛对轮状病毒接种产生局部免疫球蛋白A反应；(iv) 大多数产生轮状病毒抗体的细胞位于近端小肠的黏膜中。
