Archambault D, Morin G, Elazhary Y, Joncas J H, Roy R S
Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec, Canada.
Comp Immunol Microbiol Infect Dis. 1988;11(1):11-20. doi: 10.1016/0147-9571(88)90003-3.
Two groups of 3-month old calves were immunized intramuscularly with attenuated bovine rotavirus and boosted 21 and 42 days later. The first group of three calves were vaccinated with live virus emulsified with incomplete Freund's adjuvant (IFA) and the second group was immunized with live virus suspended in phosphate buffered saline (PBS). Three other calves, serving as controls, were inoculated with PBS emulsified with IFA. The specific cell-mediated and antibody responses of the animals were studied. Preliminary analysis of in vitro peripheral blood lymphocyte transformation to bovine rotavirus determined optimal conditions as: 96 h culture period, 5 X 10(5) cells per culture in RPMI 1640 medium containing 10% heat-inactivated bovine fetal serum and the use of inactivated virus in the cell culture at a concentration of 5 X 10(6) median tissue culture infective dose before inactivation. Specific blastic stimulation was observed on calves immunized with the rotavirus emulsified with IFA after the second and third vaccine inoculation with stimulation index values varying from 2.00 to 5.73. Serum neutralizing antibody titers of 1/25,600 were also induced in the same calves. Calves immunized with rotavirus-PBS suspension developed a mean antibody titer of 1/1,600, but showed no specific lymphocyte stimulation. No increase in specific immune responses was detected in the control animals.
将两组3月龄犊牛进行肌肉注射减毒牛轮状病毒免疫，并在21天和42天后进行加强免疫。第一组三头犊牛用不完全弗氏佐剂（IFA）乳化的活病毒进行接种，第二组用悬浮于磷酸盐缓冲盐水（PBS）中的活病毒进行免疫。另外三头犊牛作为对照，接种用IFA乳化的PBS。研究了这些动物的特异性细胞介导反应和抗体反应。对体外外周血淋巴细胞对牛轮状病毒的转化进行的初步分析确定最佳条件为：培养期96小时，在含有10%热灭活牛胎血清的RPMI 1640培养基中每个培养孔5×10⁵个细胞，并在细胞培养中使用浓度为5×10⁶半数组织培养感染剂量的灭活病毒。在用IFA乳化的轮状病毒免疫的犊牛在第二次和第三次接种疫苗后观察到特异性母细胞刺激，刺激指数值在2.00至5.73之间变化。在同一批犊牛中还诱导出了1/25,600的血清中和抗体效价。用轮状病毒-PBS悬液免疫的犊牛产生的平均抗体效价为1/1,600，但未显示出特异性淋巴细胞刺激。在对照动物中未检测到特异性免疫反应的增加。
