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合成寡脱氧核苷酸引物在克隆和测序8种流感病毒（A/PR/8/34）片段中的应用

The use of synthetic oligodeoxynucleotide primers in cloning and sequencing segment of 8 influenza virus (A/PR/8/34).

作者信息

Winter G, Fields S, Gait M J, Brownlee G G

出版信息

Nucleic Acids Res. 1981 Jan 24;9(2):237-45. doi: 10.1093/nar/9.2.237.

DOI:10.1093/nar/9.2.237
PMID:7208353
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326689/
Abstract

Complete double-stranded DNA copies of the RNA genes of the human influenza virus A/PR/8/34 have been synthesized by using two synthetic oligodeoxynucleotide primers. The gene encoding the non-structural proteins NS1 and NS2, prepared with these primers, has been cloned into the bacteriophage M13mp7 and sequenced. The sequence is compared with that from another human strain and from an avian strain.

摘要

利用两条合成的寡聚脱氧核苷酸引物合成了人类甲型流感病毒A/PR/8/34 RNA基因的完整双链DNA拷贝。用这些引物制备的编码非结构蛋白NS1和NS2的基因已被克隆到噬菌体M13mp7中并进行了测序。将该序列与来自另一个人类毒株以及一个禽毒株的序列进行了比较。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87a8/326689/39fb0695ecb4/nar00395-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87a8/326689/39fb0695ecb4/nar00395-0036-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/87a8/326689/39fb0695ecb4/nar00395-0036-a.jpg

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Nucleic Acids Res. 1980 Aug 25;8(16):3673-94. doi: 10.1093/nar/8.16.3673.
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Relationship between the messenger RNAs transcribed from two overlapping genes of influenza virus.
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J Virol. 2005 Dec;79(24):15246-57. doi: 10.1128/JVI.79.24.15246-15257.2005.
4
The N-terminal half of the influenza virus NS1 protein is sufficient for nuclear retention of mRNA and enhancement of viral mRNA translation.流感病毒NS1蛋白的N端一半对于mRNA的核内保留及病毒mRNA翻译的增强而言已足够。
Nucleic Acids Res. 1997 Nov 1;25(21):4271-7. doi: 10.1093/nar/25.21.4271.
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J Virol. 1993 May;67(5):2723-9. doi: 10.1128/JVI.67.5.2723-2729.1993.
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