Walaas S I, Aswad D W, Greengard P
Nature. 1983 Jan 6;301(5895):69-71. doi: 10.1038/301069a0.
Several mammalian neurotransmitter candidates, for example, serotonin, dopamine and noradrenaline, may exert some of their synaptic effects by regulating protein phosphorylation systems. Comparison of the regional distribution of brain phosphoproteins with neurotransmitter systems may help to identify the specific phosphoproteins involved in the functions of particular neurotransmitters. Here we report the association of one such phosphoprotein with the dopamine pathways in brain. This protein, of apparent molecular weight (MW) 32,000 (32K), seems to be present only in nervous tissue. Its regional distribution within the brain is very similar to the pattern of dopamine-containing nerve terminals; more specifically, the protein appears to be enriched in those dopaminoceptive neurones which possess D-1 receptors (dopamine receptors coupled to adenylate cyclase). The state of phosphorylation of the protein in these dopaminoceptive neurones can be regulated by both dopamine and cyclic AMP. These results suggest that the phosphoprotein may mediate certain of the trans-synaptic effects of dopamine acting on dopaminoceptive neurones.
几种哺乳动物神经递质候选物，例如血清素、多巴胺和去甲肾上腺素，可能通过调节蛋白质磷酸化系统发挥其部分突触效应。将脑磷酸化蛋白的区域分布与神经递质系统进行比较，可能有助于确定参与特定神经递质功能的特定磷酸化蛋白。在此，我们报告了一种这样的磷酸化蛋白与脑中多巴胺通路的关联。这种蛋白的表观分子量（MW）为32,000（32K），似乎仅存在于神经组织中。它在脑内的区域分布与含多巴胺神经末梢的模式非常相似；更具体地说，该蛋白似乎在那些具有D-1受体（与腺苷酸环化酶偶联的多巴胺受体）的多巴胺感受性神经元中富集。这些多巴胺感受性神经元中该蛋白的磷酸化状态可由多巴胺和环磷酸腺苷调节。这些结果表明，该磷酸化蛋白可能介导多巴胺作用于多巴胺感受性神经元的某些跨突触效应。
