Walaas S I, Greengard P
J Neurosci. 1984 Jan;4(1):84-98. doi: 10.1523/JNEUROSCI.04-01-00084.1984.
The present study documents the existence in mammalian brain of a phosphoprotein which may play a biological role in dopaminoceptive neurons. This protein has been designated DARPP-32 (dopamine- and adenosine 3':5'-monophosphate-regulated phosphoprotein-32,000). The regional distribution of DARPP-32 in the rat brain follows the general pattern of dopaminergic innervation. DARPP-32 is present in dopaminoceptive rather than dopaminergic neurons. Moreover, it appears to be concentrated in a subpopulation of dopaminoceptive cells, namely those containing D-1 receptors (dopamine receptors coupled to adenylate cyclase activation), where it is localized in cell bodies, dendrites, axons, and nerve terminals. DARPP-32 is phosphorylated in intact cells from the caudatoputamen by dopamine and by 8-bromo-cyclic adenosine 3':5'-monophosphate and in cell-free preparations by cyclic adenosine 3':5'-monophosphate-dependent protein kinase. In two accompanying papers, we report the purification and biochemical characterization of DARPP-32 (Hemmings, H.C., Jr., A.C. Nairn, D.W. Aswad, and P. Greengard (1984) J. Neurosci. 4: 99-110) and the immunocytochemical localization of this phosphoprotein (Ouimet, C.C., P. Miller, H.C. Hemmings, Jr., S.I. Walaas, and P. Greengard (1984) J. Neurosci. 4: 111-124).
本研究证明，哺乳动物大脑中存在一种磷蛋白，它可能在多巴胺感受神经元中发挥生物学作用。这种蛋白被命名为DARPP - 32（多巴胺和3':5'-环磷酸腺苷调节的磷蛋白 - 32000）。DARPP - 32在大鼠脑中的区域分布遵循多巴胺能神经支配的一般模式。DARPP - 32存在于多巴胺感受神经元而非多巴胺能神经元中。此外，它似乎集中在多巴胺感受细胞的一个亚群中，即那些含有D - 1受体（与腺苷酸环化酶激活偶联的多巴胺受体）的细胞中，它定位于细胞体、树突、轴突和神经末梢。DARPP - 32在来自尾状核壳核的完整细胞中被多巴胺和8 - 溴 - 3':5'-环磷酸腺苷磷酸化，在无细胞制剂中被环磷酸腺苷依赖性蛋白激酶磷酸化。在两篇随附论文中，我们报告了DARPP - 32的纯化和生化特性（亨明斯，H.C.，Jr.，A.C.奈恩，D.W.阿斯瓦德，和P.格林加德（1984年）《神经科学杂志》4: 99 - 110）以及这种磷蛋白的免疫细胞化学定位（乌伊梅特，C.C.，P.米勒，H.C.亨明斯，Jr.，S.I.瓦拉斯，和P.格林加德（1984年）《神经科学杂志》4: 111 - 124）。
