Hudson J, Goldstein D, Weil R
Proc Natl Acad Sci U S A. 1970 Jan;65(1):226-33. doi: 10.1073/pnas.65.1.226.
The transcription of polyoma (Py) viral RNA was studied in primary mouse kidney cell cultures (a) during normal, asynchronous infection, (b) under conditions where Py-induced DNA synthesis was inhibited with 5-fluorodeoxyuridine, and (c) after the synchronized onset of Py-induced DNA synthesis. The results obtained suggest that the bulk of Py RNA transcribed after the (asynchronous or synchronized) onset of Py-induced DNA synthesis, designated as "late" Py RNA, corresponds to the polycistronic transcript of most or all of the genetic information contained in a strand of circular Py DNA. The rate at which late Py RNA is transcribed is related to the amount of free Py DNA present in the cultures. Py-infected cultures contain a second, minor component of Py RNA (of one or possibly more species), designated as "early" Py RNA. At least some of this RNA continues to be transcribed if Py-induced DNA synthesis is inhibited with 5-fluorodeoxyuridine and, possibly, also late during normal infection.
在原代小鼠肾细胞培养物中研究了多瘤病毒（Py）RNA的转录情况：（a）在正常的异步感染过程中；（b）在使用5-氟脱氧尿苷抑制Py诱导的DNA合成的条件下；（c）在Py诱导的DNA合成同步开始之后。所获得的结果表明，在Py诱导的DNA合成（异步或同步）开始后转录的大部分Py RNA，被指定为“晚期”Py RNA，对应于环状Py DNA链中包含的大部分或所有遗传信息的多顺反子转录本。晚期Py RNA的转录速率与培养物中游离Py DNA的量有关。受Py感染的培养物含有Py RNA的第二种次要成分（一种或可能更多种），被指定为“早期”Py RNA。如果用5-氟脱氧尿苷抑制Py诱导的DNA合成，至少其中一些RNA会继续转录，并且在正常感染后期可能也是如此。
