Galanti N, Soprano K, Jonak G, Baserga R
Ann N Y Acad Sci. 1982 Dec 10;397:238-48. doi: 10.1111/j.1749-6632.1982.tb43431.x.
DNA recombinant technology and the manual microinjection technique were used to study the base sequences in the SV40 early gene coding for cell DNA replication and nucleolar activation. Sequences critical for rRNA gene activation are located between nucleotides 3826 to 3506 (0.39-0.33 m.u.). Base sequences from nucleotide 4350 to 4190 (0.49-0.46 m.u.) are required for cellular DNA replication. Major T antigen determinant is coded by a sequence extending from nucleotide 4190 to 3880 (0.46-0.42 m.u.). Considering that an increase in cell size is regularly accompanied by an increase in the cellular amount of rRNA, nucleolar genes should be a target for growth-in-size signals. Therefore, the SV40 early gene presents a domain responsible for cell growth in size, and another separate but proximate region coding for cellular DNA replication.
采用DNA重组技术和手动显微注射技术研究了SV40早期基因中编码细胞DNA复制和核仁激活的碱基序列。对rRNA基因激活至关重要的序列位于核苷酸3826至3506之间（0.39 - 0.33个图距单位）。细胞DNA复制需要核苷酸4350至4190（0.49 - 0.46个图距单位）的碱基序列。主要T抗原决定簇由从核苷酸4190延伸至3880（0.46 - 0.42个图距单位）的序列编码。考虑到细胞大小的增加通常伴随着细胞rRNA量的增加，核仁基因应该是大小生长信号的靶标。因此，SV40早期基因呈现出一个负责细胞大小生长的结构域，以及另一个单独但相邻的编码细胞DNA复制的区域。
