Mercer W E, Nelson D, Hyland J K, Croce C M, Baserga R
Virology. 1983 May;127(1):149-58. doi: 10.1016/0042-6822(83)90379-3.
The region of the SV40 large T-antigen molecule recognized by a panel of monoclonal antibodies has been determined using hybrid Adeno-SV40 viruses, and manual microinjection of cloned deletion mutants. In addition, an investigation was made of how monoclonal antibodies microinjected into the nucleus can affect the ability of the T-antigen coding gene to stimulate cell DNA synthesis. The monoclonal antibody Pab 14, that recognized the -COOH terminal half of large T, was comicroinjected into quiescent cells together with plasmid pCl-1. This plasmid contains only that part of the T-antigen coding gene that extends from nucleotide residue 120, counterclockwise to nucleotide residue 4002, and makes a truncated T antigen 33,000 in molecular weight and missing the last 435 amino acids on the -COOH terminal side. Monoclonal antibody Pab 14 did not inhibit the stimulation of cellular DNA synthesis caused by microinjection of pCl-1, although it did inhibit cell DNA synthesis induced by microinjection of pSV2G, a recombinant plasmid that contains the entire T-antigen coding gene of SV40.
利用腺病毒 - SV40杂交病毒以及对克隆的缺失突变体进行手动显微注射，已确定了一组单克隆抗体所识别的SV40大T抗原分子区域。此外，还研究了显微注射到细胞核中的单克隆抗体如何影响T抗原编码基因刺激细胞DNA合成的能力。识别大T抗原羧基末端一半区域的单克隆抗体Pab 14，与质粒pCl - 1一起显微注射到静止细胞中。该质粒仅包含T抗原编码基因中从核苷酸残基120逆时针延伸至核苷酸残基4002的部分，产生一种分子量为33,000的截短T抗原，且在羧基末端一侧缺失最后435个氨基酸。单克隆抗体Pab 14并未抑制因显微注射pCl - 1而引起的细胞DNA合成刺激，尽管它确实抑制了由显微注射pSV2G（一种包含SV40完整T抗原编码基因的重组质粒）诱导的细胞DNA合成。
