Rizzino A, Orme L S, De Larco J E
Exp Cell Res. 1983 Jan;143(1):143-52. doi: 10.1016/0014-4827(83)90116-7.
Transforming growth factors are known to induce anchorage-independent growth of non-transformed cells, and are released by a variety of cells, including MSV-transformed cells. This study demonstrates that the differentiated cells derived from F9 and PC-13 embryonal carcinoma cells, but not the parental cells themselves, respond by increased growth to several factors released by MSV-transformed cells, including partially purified sarcoma growth factor. The chemical properties of the growth-promoting activity are shown to match the chemical properties of the transforming growth factors released by MSV-transformed cells. Furthermore, F9 and PC-13 embryonal carcinoma cells, which do not respond to factors released by MSV-transformed cells, are shown to release factors with transforming growth factor activity. Based on the close relationship between mouse embryonal carcinoma cells and cells of early mouse embryos, it is suggested that molecules with transforming growth factor activity may play a role during the early stages of mammalian development.
已知转化生长因子可诱导未转化细胞进行不依赖贴壁的生长，且多种细胞均可释放转化生长因子，包括经莫洛尼氏肉瘤病毒（MSV）转化的细胞。本研究表明，源自F9和PC - 13胚胎癌细胞的分化细胞，而非其亲本细胞本身，会对MSV转化细胞释放的多种因子（包括部分纯化的肉瘤生长因子）产生反应，表现为生长增加。促生长活性的化学性质显示与MSV转化细胞释放的转化生长因子的化学性质相匹配。此外，对MSV转化细胞释放的因子无反应的F9和PC - 13胚胎癌细胞，也被证明可释放具有转化生长因子活性的因子。基于小鼠胚胎癌细胞与小鼠早期胚胎细胞之间的密切关系，有人提出具有转化生长因子活性的分子可能在哺乳动物发育的早期阶段发挥作用。
