Rowland I R, Wise A, Mallett A K
Food Chem Toxicol. 1983 Feb;21(1):25-9. doi: 10.1016/0278-6915(83)90264-8.
A fibre-free diet, or the same diet supplemented with 100 g cabbage or carrot cell-wall preparation/kg, was fed to rats for 28 days and the activities of a number of caecal microbial enzymes (azoreductase, aryl nitroreductase, beta-glucosidase, beta-glucuronidase, imidazole nitroreductase and nitrite reductase) were determined in vitro. The plant cell-wall preparations diluted the gut contents and decreased the number of bacteria per gram of caecal contents. Enzyme activities per gram of caecal contents were also decreased, with the exception of beta-glucosidase activity which was significantly increased. These plant cell-wall preparations also increased caecal size, and thereby significantly increased total activity per caecum of microbial azoreductase, aryl nitroreductase, beta-glucosidase and beta-glucuronidase. When bacterial metabolism was expressed per 10(9) bacteria, all enzyme activities were significantly increased in caecal samples from rats fed the plant cell-wall preparations. There was an overall concordance of 0.91 between all the enzymes when expressed per 10(9) bacteria, but of only 0.38 when enzyme activities were expressed per gram of caecal contents.
给大鼠喂食无纤维饮食，或喂食添加了100克卷心菜或胡萝卜细胞壁制剂/千克的相同饮食，持续28天，然后在体外测定一些盲肠微生物酶（偶氮还原酶、芳基硝基还原酶、β-葡萄糖苷酶、β-葡萄糖醛酸酶、咪唑硝基还原酶和亚硝酸还原酶）的活性。植物细胞壁制剂稀释了肠道内容物，并减少了每克盲肠内容物中的细菌数量。每克盲肠内容物的酶活性也降低了，但β-葡萄糖苷酶活性显著增加除外。这些植物细胞壁制剂还增大了盲肠尺寸，从而显著增加了微生物偶氮还原酶、芳基硝基还原酶、β-葡萄糖苷酶和β-葡萄糖醛酸酶每只盲肠的总活性。当以每10^9个细菌来表示细菌代谢时，喂食植物细胞壁制剂的大鼠盲肠样本中所有酶活性均显著增加。当以每10^9个细菌来表示时，所有酶之间的总体一致性为0.91，但当以每克盲肠内容物的酶活性来表示时，一致性仅为0.38。
