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相似文献

1
A simplified method for rapid determination of porphyrins by thin-layer chromatography.一种通过薄层色谱法快速测定卟啉的简化方法。
Br J Ind Med. 1978 Feb;35(1):61-6. doi: 10.1136/oem.35.1.61.
2
[New simplified determination of porphyrins and its application].[卟啉的新简化测定方法及其应用]
Nihon Eiseigaku Zasshi. 1975 Apr;30(1):101.
3
Separation of the coprophorphyrin isomers I and III by thin-layer chromatography.通过薄层色谱法分离粪卟啉异构体I和III。
Z Klin Chem Klin Biochem. 1975 Jul;13(7):299-304. doi: 10.1515/cclm.1975.13.7.299.
4
Coproporphyrin in urine of newborns with meconium aspiration syndrome.胎粪吸入综合征新生儿尿液中的粪卟啉
Clin Chem. 1983 Dec;29(12):2054-6.
5
Liquid-chromatographic profiles of urinary porphyrins.
Clin Chem. 1983 Mar;29(3):527-30.
6
[Porphyrin excretion in normal (author's transl)].[正常情况下的卟啉排泄(作者译)]
Rev Esp Fisiol. 1980 Mar;36(1):13-20.
7
Thin-layer chromatography of free porphyrins for diagnosis of porphyria.用于卟啉症诊断的游离卟啉薄层色谱法。
Clin Chem. 1989 Jun;35(6):1043-4.
8
Spot urine porphyrins/creatinine ratio profile of healthy Brazilian individuals adjusted for personal habits.巴西健康个体尿液卟啉/肌酐比值特征与个人习惯有关。
Braz J Med Biol Res. 2009 Aug;42(8):700-6. doi: 10.1590/s0100-879x2009005000002. Epub 2009 May 15.
9
Quantitation of red cell porphyrins by fluorescence scanning after thin-layer chromatography.薄层色谱后荧光扫描法定量红细胞卟啉
Clin Chim Acta. 1978 Oct 2;89(1):25-33. doi: 10.1016/0009-8981(78)90358-3.
10
The effects of water restriction and water loading on urinary excretion of lead, delta-aminolevulinic acid and coproporphyrin.限水和水负荷对铅、δ-氨基乙酰丙酸及粪卟啉尿排泄的影响。
Br J Ind Med. 1978 Nov;35(4):312-7. doi: 10.1136/oem.35.4.312.

本文引用的文献

1
Spectral-absorption coefficients of some porphyrins in the Soret-band region.某些卟啉在索雷特带区域的光谱吸收系数。
Biochem J. 1960 Jun;75(3):620-3. doi: 10.1042/bj0750620.
2
Mitochondrial coproporphyrinogen oxidase and protoporphyrin formation.线粒体粪卟啉原氧化酶与原卟啉的形成
J Biol Chem. 1961 Apr;236:1173-80.
3
[A simple method of determining delta-amino-levulino-dehydratase in the blood. Behavior of the enzyme in lead poisoning].[一种测定血液中δ-氨基-γ-酮戊酸脱水酶的简单方法。该酶在铅中毒中的表现]
Med Lav. 1965 Mar;56(3):199-205.
4
Thin-layer chromatography of free and esterified porphyrins on talc.游离和酯化卟啉在滑石粉上的薄层色谱分析。
J Chromatogr. 1969 Jul;42(3):389-95. doi: 10.1016/s0021-9673(01)80639-7.
5
Isolation of porphyrins from porphyria urine by preparative thin-layer chromatography.通过制备型薄层色谱法从卟啉尿症尿液中分离卟啉。
J Chromatogr. 1968 Nov 5;38(1):100-5. doi: 10.1016/0021-9673(68)85012-5.
6
A new precise and sensitive method for the assay of urine porphyrins using combined thin-layer chromatography and spectrophotofluorimetric analysis.一种采用薄层色谱法和分光荧光分析法联用测定尿卟啉的精确灵敏的新方法。
Clin Chim Acta. 1972 Jun;39(1):135-42. doi: 10.1016/0009-8981(72)90309-9.

一种通过薄层色谱法快速测定卟啉的简化方法。

A simplified method for rapid determination of porphyrins by thin-layer chromatography.

作者信息

Okuda T, Nakajima H, Yatsuki K, Amano M, Umeda G

出版信息

Br J Ind Med. 1978 Feb;35(1):61-6. doi: 10.1136/oem.35.1.61.

DOI:10.1136/oem.35.1.61
PMID:629891
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1008326/
Abstract

Free porphyrins, especially copro-, uro-, and protoporphyrin, were successfully separated by talc thin-layer chromatography (TLC), and were determined from the relationship of the weight and the area of porphyrins in the spot on the thin-layer plate. The results obtained from both the solvent extraction and the simple, rapid TLC method reported in this paper showed good correlation for coproporphyrin levels up to 400 microgram/1. The TLC method was applied to the determination of urinary coproporphyrin in lead workers and in rats.

摘要

游离卟啉,尤其是粪卟啉、尿卟啉和原卟啉,通过滑石粉薄层色谱法(TLC)成功分离,并根据薄层板上斑点中卟啉的重量与面积关系进行测定。本文报道的溶剂萃取法和简单快速的TLC法所获得的结果显示,对于高达400微克/升的粪卟啉水平,两者具有良好的相关性。TLC法被应用于铅作业工人和大鼠尿中粪卟啉的测定。