Okuda T, Nakajima H, Yatsuki K, Amano M, Umeda G
Br J Ind Med. 1978 Feb;35(1):61-6. doi: 10.1136/oem.35.1.61.
Free porphyrins, especially copro-, uro-, and protoporphyrin, were successfully separated by talc thin-layer chromatography (TLC), and were determined from the relationship of the weight and the area of porphyrins in the spot on the thin-layer plate. The results obtained from both the solvent extraction and the simple, rapid TLC method reported in this paper showed good correlation for coproporphyrin levels up to 400 microgram/1. The TLC method was applied to the determination of urinary coproporphyrin in lead workers and in rats.
游离卟啉,尤其是粪卟啉、尿卟啉和原卟啉,通过滑石粉薄层色谱法(TLC)成功分离,并根据薄层板上斑点中卟啉的重量与面积关系进行测定。本文报道的溶剂萃取法和简单快速的TLC法所获得的结果显示,对于高达400微克/升的粪卟啉水平,两者具有良好的相关性。TLC法被应用于铅作业工人和大鼠尿中粪卟啉的测定。