Homan E J, Easterday B C
Am J Vet Res. 1983 Feb;44(2):309-13.
Latent bovine herpesvirus-1 (BHV-1) infection was established in 6 calves and was demonstrated by reinduction of virus shedding after administration of corticosteroids. Latently infected calves failed to transmit BHV-1 during 4 weeks' contact with sentinel calves. Infected calves were killed and necropsied during latency or induced recrudescence. The BHV-1 DNA was demonstrated intranuclearly in trigeminal ganglion neurons by in situ hybridization. The BHV-1 antigen was demonstrated by immunofluorescence in trigeminal ganglion neurons during recrudescence. By electron microscopy, changes in the appearance of the Nissl bodies and a high frequency of nuclear bodies were observed in trigeminal ganglion neurons.
在6头犊牛中建立了潜伏性牛疱疹病毒1型（BHV-1）感染，通过给予皮质类固醇后病毒再次排出得以证实。在与哨兵犊牛接触的4周内，潜伏感染的犊牛未能传播BHV-1。在潜伏期或诱导复发期间，对感染的犊牛实施安乐死并进行尸检。通过原位杂交在三叉神经节神经元的细胞核内检测到BHV-1 DNA。在复发期间，通过免疫荧光在三叉神经节神经元中检测到BHV-1抗原。通过电子显微镜观察，在三叉神经节神经元中观察到尼氏体外观的变化和高频率的核小体。
