Charman H P, Kim N, White M, Marquardt H, Gilden R V, Kawakami T
J Natl Cancer Inst. 1975 Dec;55(6):1419-24. doi: 10.1093/jnci/55.6.1419.
Using sensitive radiommunoprecipitation assays for highly purified type-C RNA tumor virus proteins, we found that 5 of 16 clinically normal gibbons (including 4 of 5 normal animals from a colony with 2 cases of lymphoma) and 4 of 4 experimentally inoculated gibbons formed antibodies to the major structural protein (p30) of gibbon ape leukemia virus (GaLV). An additional woolly monkey immunized with the closely related simian sarcoma virus also formed antibodies detectable with GaLV p30. Of 20 patients immunized with formalin-inactivated Rauscher murine leukemia virus (R-MuLV), 10 were previously reported to have antibodies to MuLV as determined by an internally labeled banded virus radioimmunoprecipitation assay. In comparison studies with purified R-MuLV proteins, 7 of 20 patients formed antibodies: 3/20 to R-MuLV p30 only, 1/20 to R-MuLV glycoprotein (gp) 70 only, and 3/20 to both p30 and gp70. Most responders were melanoma patients receiving immunotherapy with BCG. Additionally, rhesus monkeys produced antibodies to the endogenous cat virus RD114 and closely related endogenous baboon leukemia virus p30's. Thus these studies demonstrated the ability of primates (including humans) to form antibodies to well-characterized proteins from endogenous and exogenous type-C viruses and the potential utility of these assays for seroepidemiologic studies.
利用针对高度纯化的C型RNA肿瘤病毒蛋白的敏感放射免疫沉淀试验，我们发现，16只临床正常的长臂猿中有5只（包括来自一个有2例淋巴瘤病例的群体中的5只正常动物中的4只）以及4只经实验接种的长臂猿产生了针对长臂猿白血病病毒（GaLV）主要结构蛋白（p30）的抗体。另外，一只用密切相关的猿猴肉瘤病毒免疫的绒毛猴也产生了可用GaLV p30检测到的抗体。在20名用福尔马林灭活的劳舍尔小鼠白血病病毒（R-MuLV）免疫的患者中，先前有报告称，通过内部标记的带状病毒放射免疫沉淀试验确定，其中10名患者有针对MuLV的抗体。在与纯化的R-MuLV蛋白的比较研究中，20名患者中有7名产生了抗体：仅3/20针对R-MuLV p30，仅1/20针对R-MuLV糖蛋白（gp）70，3/20针对p30和gp70两者。大多数有反应者是接受卡介苗免疫治疗的黑色素瘤患者。此外，恒河猴产生了针对内源性猫病毒RD114和密切相关的内源性狒狒白血病病毒p30的抗体。因此，这些研究证明了灵长类动物（包括人类）产生针对内源性和外源性C型病毒特征明确的蛋白的抗体的能力，以及这些试验在血清流行病学研究中的潜在用途。
