Monboisse J C, Braquet P, Randoux A, Borel J P
Biochem Pharmacol. 1983 Jan 1;32(1):53-8. doi: 10.1016/0006-2952(83)90651-2.
Calf skin acid-soluble collagen in microfibrillar form was incubated with free oxygen radicals produced by the system xanthine oxidase + hypoxanthine. This incubation liberated peptides of a size smaller than that of alpha-chains, as demonstrated by SDS-PAGE and by evaluation of the 4-hydroxyproline contained in small peptides. The amount of liberated peptides was found to increase with time. The process was inhibited by addition of superoxide dismutase to the medium but not by addition of catalase. Two flavonoids extracted from bilberries and a third one from grapes were demonstrated to protect collagen against this non-enzymatic proteolytic activity. This work confirms that collagen may be degraded during the process of inflammation and that some flavonoids are endowed with protective properties.
将微纤维形式的小牛皮酸溶性胶原蛋白与黄嘌呤氧化酶+次黄嘌呤系统产生的游离氧自由基一起孵育。如SDS-PAGE和对小肽中所含4-羟基脯氨酸的评估所示，这种孵育释放出比α链尺寸更小的肽。发现释放的肽量随时间增加。该过程通过向培养基中添加超氧化物歧化酶而受到抑制，但添加过氧化氢酶则无此作用。从越橘中提取的两种黄酮类化合物和从葡萄中提取的第三种黄酮类化合物被证明可保护胶原蛋白免受这种非酶促蛋白水解活性的影响。这项工作证实胶原蛋白可能在炎症过程中被降解，并且一些黄酮类化合物具有保护特性。
