Dietary influences on excretory pathways and tissue residues of zearalenone and zearalenols in the rat.

Experiments were conducted with male weanling rats to determine the effect of high dietary protein (40% casein), alfalfa (25%), protein + alfalfa (25% casein + 25% alfalfa), or anion-exchange resin (5%) on the relative proportions of free and conjugated zearalenone and zearalenols excreted in urine and feces following a single oral dose of zearalenone. About 90% of the dose was excreted in feces after 48 h while the remaining 10% was excreted in urine. Zearalenone and metabolites were excreted mainly in free form with conjugates being found only in urine. Rats fed high-protein diets excreted more free zearalenone and alpha-zearalenol in urine than did controls (16.3% casein). The feeding of protein + alfalfa also resulted in increased urinary losses of free zearalenone. A reduction in urinary losses of conjugated zearalenone and alpha-zearalenol was seen when 5% anion-exchange resin was fed. Fecal excretion of zearalenone and alpha- and beta-zearalenol increased when alfalfa + protein was fed. Residual zearalenone and zearalenols were measured in liver and kidney 18 h after dosing. All treatments except high protein reduced residues of zearalenone and alpha-zearalenol in liver when compared with controls. Renal residues of zearalenone were lowered only by supplements of protein + alfalfa or anion-exchange resin. Each of the treatments fed has been shown to reduce zearalenone toxicosis in rats, but it was concluded that each does so by a different mechanism.