Canaani O D, Sauer K
Biochim Biophys Acta. 1978 Mar 13;501(3):545-51. doi: 10.1016/0005-2728(78)90120-2.
The absorption and CD spectra of chloroplast fragments from spinach, barley and a barley mutant (chlorophyll b-minus) were studied at temperatures of 23 degrees C and --196 degrees C. The CD spectrum of wild type barley and spinach at --196 degrees C showed troughs at 640, 653, 676 and 695 nm and a maximum at 667 nm. The CD spectrum of the barley mutant at --196 degrees C consisted of a large trough at 684 nm, a small trough at 695 nm and a positive peak at 670 nm. A new feature observed at --196 degrees C but not at 23 degrees C is the trough at 640 nm. This 640 nm CD signal is missing in the CD spectrum of the barley mutant. It is attributable to the light-harvesting chlorophyll a/b protein which appears to be missing in the mutant. Another new feature, the trough at 695 nm, was observed in the CD spectra of spinach, barley and the barley mutant at --196 degrees C. The 695 nm trough appears to be sensitive to detergents and it may be due to a labile chlorophyll a.protein complex. Possible interpretations of the data are discussed.
在23摄氏度和零下196摄氏度的温度下，研究了菠菜、大麦及其一个大麦突变体（叶绿素b缺失型）的叶绿体片段的吸收光谱和圆二色光谱。野生型大麦和菠菜在零下196摄氏度时的圆二色光谱在640、653、676和695纳米处有波谷，在667纳米处有一个峰值。大麦突变体在零下196摄氏度时的圆二色光谱由684纳米处的一个大波谷、695纳米处的一个小波谷和670纳米处的一个正峰组成。在零下196摄氏度时观察到但在23摄氏度时未观察到的一个新特征是640纳米处的波谷。在大麦突变体的圆二色光谱中没有这个640纳米的圆二色信号。它归因于捕光叶绿素a/b蛋白，该蛋白在突变体中似乎缺失。另一个新特征，即695纳米处的波谷，在菠菜、大麦和大麦突变体在零下196摄氏度时的圆二色光谱中被观察到。695纳米处的波谷似乎对去污剂敏感，它可能是由于一种不稳定的叶绿素a-蛋白复合物。讨论了对这些数据的可能解释。
