Toyoshima K, Yoshida M, Kawai S, Kitamura N, Yamamoto T, Segawa K
Princess Takamatsu Symp. 1982;12:275-83.
The Y73 strain of avian sarcoma virus isolated from a transplantable chicken tumor was defective in its replicating capacity. The virus caused sarcoma but not acute leukosis in chickens even when inoculated intravenously. It induced transformed cell-foci in cultured fibroblasts and the viral genome responsible for in vitro transformation was 26S RNA. The RNA was composed of sequences in common with helper virus RNA and Y73-specific sequence. The specific sequence "yes" did not hybridize with complementary DNA to the src gene of Rous sarcoma virus (cDNAsrc) and it had a unique counterpart in normal cell DNA. The yes gene was located in the middle of the 26S genome and the sequences common to the helper virus were located toward both ends. The 26S RNA coded for an polyprotein of 90,000 daltons (p90) which included p19 of viral core proteins in addition to the polypeptide unique to the yes gene. p90 had protein kinase activity specific for tyrosine residue and itself could be phosphorylated at tyrosine residue in vivo and in vitro, and at serine residue in vivo.
从一种可移植性鸡肿瘤中分离出的禽肉瘤病毒Y73株，其复制能力存在缺陷。该病毒即使经静脉接种，也只会在鸡身上引发肉瘤，而不会导致急性白血病。它能在培养的成纤维细胞中诱导转化细胞灶形成，负责体外转化的病毒基因组是26S RNA。该RNA由与辅助病毒RNA共有的序列以及Y73特异性序列组成。“yes”特异性序列不与劳氏肉瘤病毒src基因的互补DNA（cDNAsrc）杂交，并在正常细胞DNA中有独特的对应序列。yes基因位于26S基因组的中间，辅助病毒共有的序列位于两端。26S RNA编码一种90,000道尔顿的多聚蛋白（p90），除yes基因特有的多肽外，还包括病毒核心蛋白的p19。p90具有对酪氨酸残基特异的蛋白激酶活性，其自身在体内和体外均可在酪氨酸残基处磷酸化，在体内还可在丝氨酸残基处磷酸化。
