噬菌体P22基因组上额外的限制性内切酶切割位点。
Additional restriction endonuclease cleavage sites on the bacteriophage P22 genome.
作者信息
Casjens S, Hayden M, Jackson E, Deans R
出版信息
J Virol. 1983 Feb;45(2):864-7. doi: 10.1128/JVI.45.2.864-867.1983.
Abstract
We present complete restriction endonuclease cleavage site maps of the bacteriophage P22 chromosome for 16 enzymes with six base recognition sequences, thereby positioning 116 new sites on the chromosome. Twenty-four such restriction maps for P22 DNA, containing 162 sites, have now been completed, and three enzymes were found that did not cut P22 DNA. Our results are consistent with the ideas that ClaI does not cleave the methylated recognition sequence ATCGA(me)T or A(me)TCGAT and StuI does not cleave the methylated recognition sequence AGGCC(me)T.
摘要
我们展示了噬菌体P22染色体对于16种具有六碱基识别序列的限制性内切酶的完整切割位点图谱，从而在染色体上定位了116个新位点。目前已完成24张这样的P22 DNA限制性图谱，包含162个位点，并且发现有三种酶不能切割P22 DNA。我们的结果与以下观点一致：ClaI不能切割甲基化的识别序列ATCGA(me)T或A(me)TCGAT，而StuI不能切割甲基化的识别序列AGGCC(me)T。
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