Kanda T, Shibuta H
Microbiol Immunol. 1982;26(11):1045-55. doi: 10.1111/j.1348-0421.1982.tb00253.x.
It was previously shown that a temperature-sensitive mutant of Sendai virus, ts-23, readily establishes persistent infection in Vero cells at 37 C, a permissive temperature for growth of the mutant. In the present study, it was demonstrated that the virus yield from ts-23-infected Vero cells at 37 C began to decrease 48 to 72 hr postinfection, after an initial phase of high virus production. Before the decrease in virus production, the formation of viral nucleoprotein declined, although synthesis of all species of viral protein continued. It was suggested that the limited formation of viral nucleoprotein and the decrease in virus production were due to the restriction of viral RNA synthesis which began to occur early after infection in ts-23-infected cells at 37 C. The mutant has a temperature-sensitive defect in RNA polymerase activity and the temperature 37 C, used for establishment of persistent infection, would be a semi-permissive temperature for the RNA polymerase activity of the mutant. The ts-23 mutant interfered with the replication of the parental wild virus in Vero cells at 37 C.
先前的研究表明，仙台病毒的温度敏感突变体ts-23在37℃（该突变体生长的允许温度）下能轻易地在Vero细胞中建立持续感染。在本研究中，已证明在37℃下，ts-23感染的Vero细胞产生的病毒产量在感染后48至72小时开始下降，此前有一个病毒高产量的初始阶段。在病毒产量下降之前，病毒核蛋白的形成减少，尽管所有种类的病毒蛋白合成仍在继续。有人提出，病毒核蛋白形成受限和病毒产量下降是由于在37℃下ts-23感染的细胞感染后早期开始出现的病毒RNA合成受到限制。该突变体在RNA聚合酶活性方面存在温度敏感缺陷，而用于建立持续感染的37℃温度对该突变体的RNA聚合酶活性而言是一个半允许温度。ts-23突变体在37℃下干扰了亲代野生病毒在Vero细胞中的复制。
