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Post-transcriptional modification of the wobble nucleotide in anticodon-substituted yeast tRNAArgII after microinjection into Xenopus laevis oocytes.

作者信息

Fournier M, Haumont E, de Henau S, Gangloff J, Grosjean H

出版信息

Nucleic Acids Res. 1983 Feb 11;11(3):707-18. doi: 10.1093/nar/11.3.707.

DOI:10.1093/nar/11.3.707
PMID:6300762
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC325747/
Abstract

An enzymatic procedure for the replacement of the ICG anticodon of yeast tRNAArgII by NCG trinucleotide (N = A, C, G or U) is described. Partial digestion with S1-nuclease and T1-RNAase provides fragments which, when annealed together, form an "anticodon-deprived" yeast tRNAArgII. A novel anticodon, phosphorylated with (32P) label on its 5' terminal residue, is then inserted using T4-RNA ligase. Such "anticodon-substituted" yeast tRNAArgII are microinjected into the cytoplasm of Xenopus laevis oocytes and shown to be able to interact with the anticodon maturation enzymes under in vivo conditions. Our results indicate that when adenosine occurs in the wobble position (A34) in yeast tRNAArgII it is efficiently modified into inosine (I34) while uridine (U34) is transformed into two uridine derivatives, one of which is probably mcm5U. In contrast, when a cytosine (C34) or guanosine (G34) occurs, they are not modified. These results are at variance with those obtained previously under similar conditions with anticodon derivatives of yeast tRNAAsp harbouring A, C, G or U as the first anticodon nucleotide. In this case, guanosine and uridine were modified while adenosine and cytosine were not.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/405a/325747/d6984fa04fe0/nar00348-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/405a/325747/94cb04519936/nar00348-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/405a/325747/d6984fa04fe0/nar00348-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/405a/325747/94cb04519936/nar00348-0165-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/405a/325747/d6984fa04fe0/nar00348-0167-a.jpg

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1
Post-transcriptional modification of the wobble nucleotide in anticodon-substituted yeast tRNAArgII after microinjection into Xenopus laevis oocytes.
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2
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引用本文的文献

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2
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3
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本文引用的文献

1
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EMBO J. 1982;1(3):291-5. doi: 10.1002/j.1460-2075.1982.tb01162.x.
2
Differentiation of cells of the Rana pipiens gastrula in unconditioned medium.爪蟾原肠胚细胞在未 conditioned 培养基中的分化。 注：这里原文中“unconditioned medium”的“conditioned”可能拼写有误，正常应该是“conditioned medium”，意思是“条件培养基”，如果是“unconditioned medium”就是“非条件培养基”，但仅根据现有文本准确翻译就是上述译文。
J Embryol Exp Morphol. 1959 Jun;7:210-22.
3
酵母苯丙氨酸tRNA反密码子相邻的3'位鸟苷向N1-甲基鸟苷和y核苷的酶促转化：取决于反密码子序列。
EMBO J. 1987 Feb;6(2):477-83. doi: 10.1002/j.1460-2075.1987.tb04778.x.
4
Enzymatic 2'-O-methylation of the wobble nucleoside of eukaryotic tRNAPhe: specificity depends on structural elements outside the anticodon loop.
EMBO J. 1986 May;5(5):1105-9. doi: 10.1002/j.1460-2075.1986.tb04329.x.
5
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Nucleic Acids Res. 1988 Dec 23;16(24):11591-606. doi: 10.1093/nar/16.24.11591.
6
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EMBO J. 1992 Nov;11(11):4159-65. doi: 10.1002/j.1460-2075.1992.tb05509.x.
Mutants of Su+7 tRNA include a functional tRNA with an altered T pseudo uracil CG sequence.
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Cell. 1981 Sep;25(3):815-23. doi: 10.1016/0092-8674(81)90189-6.
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J Biol Chem. 1982 Apr 25;257(8):4514-21.
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10
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