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秀丽隐杆线虫四个tRNA基因的序列以及秀丽隐杆线虫tRNALeu（反密码子IAG）在非洲爪蟾卵母细胞中的表达。

Sequences of four tRNA genes from Caenorhabditis elegans and the expression of C. elegans tRNALeu (anticodon IAG) in Xenopus oocytes.

作者信息

Tranquilla T A, Cortese R, Melton D, Smith J D

出版信息

Nucleic Acids Res. 1982 Dec 20;10(24):7919-34. doi: 10.1093/nar/10.24.7919.

DOI:10.1093/nar/10.24.7919
PMID:6761649
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC327059/
Abstract

Four tRNA genes have been identified in cloned segments of Caenorhabditis elegans DNA by tRNA hybridisation and expression after injection into Xenopus laevis oocyte nuclei. From DNA sequencing these are (with DNA anticodon sequences) tRNAAsp (GTC), tRNALeu (AAG), tRNALys (CTT) and tRNAPro (TGG). Their flanking DNA sequences are compared. Two identical tRNALys (CTT) genes from different regions of the genome have quite unrelated 5' flanking sequences. The tRNA synthesised in Xenopus oocytes after injection of the tRNALeu cloned DNA has the modified anticodon IAG. The tRNALeu gene precursor transcript from injected oocytes has short 5' and 3' additional sequences and lacks certain of those modified bases found in the processed tRNA.

摘要

通过tRNA杂交以及将其注射到非洲爪蟾卵母细胞核后进行表达，在秀丽隐杆线虫DNA的克隆片段中鉴定出了四个tRNA基因。通过DNA测序，它们分别是（带有DNA反密码子序列）天冬氨酸tRNA（GTC）、亮氨酸tRNA（AAG）、赖氨酸tRNA（CTT）和脯氨酸tRNA（TGG）。对它们两侧的DNA序列进行了比较。来自基因组不同区域的两个相同的赖氨酸tRNA（CTT）基因具有完全不相关的5'侧翼序列。注射亮氨酸tRNA克隆DNA后在非洲爪蟾卵母细胞中合成的tRNA具有修饰后的反密码子IAG。注射卵母细胞后的亮氨酸tRNA基因前体转录本具有短的5'和3'附加序列，并且缺少加工后的tRNA中发现的某些修饰碱基。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21a7/327059/69f544ccb276/nar00393-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21a7/327059/53946c19460c/nar00393-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21a7/327059/02befe0dbda1/nar00393-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21a7/327059/6b0d1e553dde/nar00393-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21a7/327059/69f544ccb276/nar00393-0045-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21a7/327059/53946c19460c/nar00393-0040-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21a7/327059/02befe0dbda1/nar00393-0041-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21a7/327059/6b0d1e553dde/nar00393-0044-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21a7/327059/69f544ccb276/nar00393-0045-a.jpg

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本文引用的文献

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A split promoter for a eucaryotic tRNA gene.一种真核生物tRNA基因的分裂启动子。
Cell. 1981 May;24(2):573-85. doi: 10.1016/0092-8674(81)90348-2.
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Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.
与粗糙脉孢菌QA基因簇相邻的一个亮氨酸tRNA基因。
Nucleic Acids Res. 1984 Jul 25;12(14):5757-65. doi: 10.1093/nar/12.14.5757.
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