Vacher J, Grosjean H, de Henau S, Finelli J, Buckingham R H
Eur J Biochem. 1984 Jan 2;138(1):77-81. doi: 10.1111/j.1432-1033.1984.tb07883.x.
In this paper we describe the construction of a yeast tRNACys UGA suppressor. After specific hydrolysis of the parent molecule, the first base of the anticodon GCA was replaced by a uracil. The resulting molecule, harboring a UCA anticodon, was injected into Xenopus laevis oocytes in order to test its biological activities. The level of aminoacylation was similar to that of the parent molecule. Readthrough of the UGA termination codon in beta-globin mRNA, coinjected with the tRNA, indicated suppressor activity; however, tRNACys (anticodon UCA) was a much less efficient suppressor than others tested under the same conditions. We see no post-transcriptional modification of the uracil in the anticodon wobble position after injection into oocytes. This may be related to the low suppressor activity; however, it is also possible that other features of tRNACys structure may be unadapted to efficient UCA anticodon function.
在本文中，我们描述了酵母tRNACys UGA抑制子的构建。在对亲本分子进行特异性水解后，反密码子GCA的第一个碱基被尿嘧啶取代。将所得的带有UCA反密码子的分子注射到非洲爪蟾卵母细胞中，以测试其生物学活性。氨酰化水平与亲本分子相似。与该tRNA共注射的β-珠蛋白mRNA中UGA终止密码子的通读表明了抑制子活性；然而，在相同条件下测试时，tRNACys（反密码子UCA）作为抑制子的效率远低于其他测试对象。在注射到卵母细胞后，我们未观察到反密码子摆动位置的尿嘧啶有转录后修饰。这可能与抑制子活性低有关；然而，也有可能是tRNACys结构的其他特征不适合UCA反密码子的高效功能。
