Anderson K M, Baranowski J, Bonomi P, Economou S G
Prostate. 1983;4(1):83-9. doi: 10.1002/pros.2990040108.
The distribution of proteins in samples from 8 prostate and 4 colon adenocarcinomas and 1 hepatoma was analyzed by 2-dimensional protein electrophoresis. Composite "normograms" based upon their distribution of proteins were developed from these patterns. Particular attention was paid to acidic proteins between pI 3.5 and 5.9. Of 161 proteins enumerated, 23 of the first 135 were present in prostate cancers, compared with 68 in colon cancer and 85 in the hepatoma. The 26 proteins denoted from nos. 135 to 161 were prostate associated, and none was evident in the colon or hepatoma samples. Twenty-seven prostate, 20 colon, and 48 liver cancer proteins were "unique" to each of the 3 cancers, respectively. The patterns of protein associated with each type of cancer were so dissimilar that with this technique no difficulty should be experienced in distinguishing these carcinomas originating from 3 different types of "stem" cells without obligatory recourse to microscopy.
通过二维蛋白质电泳分析了来自8例前列腺癌、4例结肠癌和1例肝癌样本中的蛋白质分布情况。根据这些蛋白质分布模式绘制了复合“正常图谱”。特别关注了pH值在3.5至5.9之间的酸性蛋白质。在列出的161种蛋白质中，前135种蛋白质里有23种存在于前列腺癌中，相比之下，结肠癌中有68种，肝癌中有85种。编号为135至161的26种蛋白质与前列腺相关，在结肠癌或肝癌样本中均未发现。分别有27种前列腺癌、20种结肠癌和48种肝癌蛋白质是这三种癌症各自特有的。与每种癌症类型相关的蛋白质模式差异很大，以至于使用这项技术在区分源自3种不同类型“干细胞”的这些癌症时，无需借助显微镜就不会遇到困难。
