Lestrovaia N N, Lebedeva Zh D, Volkova I M, Aren A K, Bukbarde V U
Prikl Biokhim Mikrobiol. 1983 Jan-Feb;19(1):130-5.
The possibility of purifying lipase from Geotrichum asteroides by chromatography with a modified silochrome used as sorbent has been explored. The paper presents a scheme of adsorption and subsequent desorption of lipase from octylsilochrome which requires that ethylene glycol, Na-deoxycholate and Triton X-100 be used as eluents. Triton, the last in the sequence, eluates the major portion of the enzyme. This eluent can be removed from the lipase solution by means of ultrafiltration through the Ripore-4 membrane in the presence of glycerol.
探索了使用改性硅铬作为吸附剂通过色谱法从星状地霉中纯化脂肪酶的可能性。本文提出了脂肪酶从辛基硅铬上吸附及随后解吸的方案，该方案要求使用乙二醇、脱氧胆酸钠和 Triton X-100 作为洗脱剂。序列中的最后一种洗脱剂 Triton 能洗脱大部分酶。在甘油存在的情况下，可通过 Ripore-4 膜超滤从脂肪酶溶液中除去这种洗脱剂。
