Marchioli C C, Yancey R J, Petrovskis E A, Timmins J G, Post L E
Upjohn Company, Kalamazoo, Michigan 49001.
J Virol. 1987 Dec;61(12):3977-82. doi: 10.1128/JVI.61.12.3977-3982.1987.
Pseudorabies virus (PRV) is an alphaherpesvirus which causes an economically important disease of swine. One of the PRV glycoproteins, gp50, was previously identified as the sequence homolog of herpes simplex virus glycoprotein gD (E.A. Petrovskis, J.G. Timmins, M.A. Armentrout, C.C. Marchioli, R.J. Yancey, Jr., and L.E. Post, J. Virol. 59:216-223, 1986). gp50 was evaluated as a PRV subunit vaccine candidate. gp50 protected mice from PRV-induced mortality either when delivered via infection with a recombinant vaccinia virus or when administered as a subunit vaccine produced in a eucaryotic cell line, Chinese hamster ovary (CHO) cells. In addition, gp50 synthesized in CHO cells protected pigs from lethal infection with PRV. This result demonstrates that a single viral glycoprotein could induce a protective immune response in the natural host of a herpesvirus infection.
伪狂犬病病毒（PRV）是一种甲型疱疹病毒，可引发对养猪业具有重要经济影响的疾病。PRV的一种糖蛋白gp50，先前被鉴定为单纯疱疹病毒糖蛋白gD的序列同源物（E.A. 彼得罗夫斯基、J.G. 廷明斯、M.A. 阿门特劳特、C.C. 马尔乔利、R.J. 扬西、小R.J. 扬西和L.E. 波斯特，《病毒学杂志》59:216 - 223，1986年）。gp50被评估为PRV亚单位疫苗候选物。当通过重组痘苗病毒感染递送gp50时，或者当作为在真核细胞系中国仓鼠卵巢（CHO）细胞中产生的亚单位疫苗给药时，gp50可保护小鼠免受PRV诱导的死亡。此外，在CHO细胞中合成的gp50可保护猪免受PRV的致死性感染。这一结果表明，单一病毒糖蛋白可在疱疹病毒感染的天然宿主中诱导保护性免疫反应。
