Sofranková A, Dockray G J
Am J Physiol. 1983 Apr;244(4):G370-4. doi: 10.1152/ajpgi.1983.244.4.G370.
The influence of the endocrine pancreas on exocrine pancreatic function was studied in rats made diabetic by administration of streptozotocin. In normal urethan-anesthetized rats the C-terminal octapeptide of cholecystokinin (CCK-8) stimulated the rate of flow and rates of amylase and trypsinogen release from the pancreas; the proportions of the two enzymes did not change with repeated stimulation. In diabetic rats CCK-8 (0.3-1.2 nmol . kg-1 iv) stimulated significantly greater increases in trypsinogen output and rate of flow compared with normal animals. In contrast, amylase responses to CCK-8 were significantly depressed in diabetic rats. There were no differences between normal and diabetic rats in the flow and total protein responses to exogenous secretin. The present results indicate that the effects of insulin deprivation on relative rates of enzyme synthesis are also expressed in the secretion of the intact pancreas in vivo. These results also raise the possibility that in the normal intact gland there may be nonparallel release of enzymes at different rates of secretion depending on the relative secretory activities of cells in the immediate vicinity of the islets of Langerhans and those distant to the islets.
通过给大鼠注射链脲佐菌素使其患糖尿病，研究内分泌胰腺对外分泌胰腺功能的影响。在正常经乌拉坦麻醉的大鼠中，胆囊收缩素的C末端八肽（CCK - 8）可刺激胰腺的流速以及淀粉酶和胰蛋白酶原的释放速率；重复刺激时两种酶的比例不变。与正常动物相比，在糖尿病大鼠中，CCK - 8（0.3 - 1.2 nmol·kg⁻¹静脉注射）显著刺激了胰蛋白酶原输出和流速的更大增加。相比之下，糖尿病大鼠中淀粉酶对CCK - 8的反应明显受到抑制。正常大鼠和糖尿病大鼠对外源促胰液素的流速和总蛋白反应没有差异。目前的结果表明，胰岛素缺乏对酶合成相对速率的影响也体现在体内完整胰腺的分泌中。这些结果还提出了一种可能性，即在正常完整的腺体中，根据胰岛附近细胞和远离胰岛细胞的相对分泌活动，可能会以不同的分泌速率非平行释放酶。
