Wikman-Coffelt J, Sievers R, Coffelt R J, Parmley W W
Biochem Biophys Res Commun. 1983 Mar 16;111(2):450-5. doi: 10.1016/0006-291x(83)90327-3.
Oscillations of cAMP with the cardiac cycle were demonstrated in the rat heart using a stimulator-triggered rapid freeze-clamp to decrease the temperature of the heart from 37 degrees C to -80 degrees C in 5 msec (20,000 degrees/sec) at a predetermined phase of the cardiac cycle. The nucleotide, cAMP, oscillated 60% with the cardiac cycle during normal working conditions, the higher cAMP value occurring during systole.
在大鼠心脏中，通过刺激触发的快速冷冻钳夹技术，在心动周期的预定阶段将心脏温度从37摄氏度在5毫秒内（每秒20,000摄氏度）降至-80摄氏度，从而证明了cAMP随心动周期的振荡。在正常工作条件下，核苷酸cAMP随心动周期振荡60%，cAMP值在收缩期较高。
