同一乙酰胆碱受体通道的连续开放在开放时间上具有相关性。
Successive openings of the same acetylcholine receptor channel are correlated in open time.
作者信息
Jackson M B, Wong B S, Morris C E, Lecar H, Christian C N
出版信息
Biophys J. 1983 Apr;42(1):109-14. doi: 10.1016/S0006-3495(83)84375-6.
Abstract
Previous analysis of single-channel current records has shown that both the opening and closing transitions of chemically activated ion channels are operated by fast and slow kinetic processes. The fast component in the kinetics of channel opening has been interpreted as the reopening of a channel that has just closed. The fast component in the kinetics of channel closure has many possible explanations and is therefore more difficult to interpret. We can gain insight into the closing process by asking whether the lifetimes of successive openings of an acetylcholine receptor channel are correlated in open-state lifetime. Five kinetic models of channel closure are considered. Two of these models predict uncorrelated open-state lifetimes, one predicts correlated open-state lifetimes, and for two others a range of behavior is possible. Acetylcholine receptor channel data from cultured rat muscle are analyzed to show that open-state lifetimes are correlated, eliminating two models of channel gating.
摘要
先前对单通道电流记录的分析表明，化学激活离子通道的开放和关闭转变均由快速和慢速动力学过程控制。通道开放动力学中的快速成分被解释为刚关闭的通道的重新开放。通道关闭动力学中的快速成分有多种可能的解释，因此更难解读。通过询问乙酰胆碱受体通道连续开放的寿命在开放状态寿命方面是否相关，我们可以深入了解关闭过程。考虑了通道关闭的五种动力学模型。其中两种模型预测开放状态寿命不相关，一种预测开放状态寿命相关，另外两种则可能有一系列行为。对来自培养大鼠肌肉的乙酰胆碱受体通道数据进行分析，结果表明开放状态寿命是相关的，从而排除了两种通道门控模型。
相似文献
1
Successive openings of the same acetylcholine receptor channel are correlated in open time.同一乙酰胆碱受体通道的连续开放在开放时间上具有相关性。
Biophys J. 1983 Apr;42(1):109-14. doi: 10.1016/S0006-3495(83)84375-6.
2
Acetylcholine receptor kinetics. A description from single-channel currents at snake neuromuscular junctions.乙酰胆碱受体动力学。来自蛇神经肌肉接头单通道电流的描述。
Biophys J. 1982 Sep;39(3):253-61. doi: 10.1016/S0006-3495(82)84515-3.
3
Kinetics of unliganded acetylcholine receptor channel gating.未结合配体的乙酰胆碱受体通道门控动力学。
Biophys J. 1986 Mar;49(3):663-72. doi: 10.1016/S0006-3495(86)83693-1.
4
Dependence of acetylcholine receptor channel kinetics on agonist concentration in cultured mouse muscle fibres.培养的小鼠肌纤维中乙酰胆碱受体通道动力学对激动剂浓度的依赖性。
J Physiol. 1988 Mar;397:555-83. doi: 10.1113/jphysiol.1988.sp017019.
5
Open channel noise. II. A test for coupling between current fluctuations and conformational transitions in the acetylcholine receptor.开放通道噪声。II. 乙酰胆碱受体中电流波动与构象转变之间耦合的测试。
Biophys J. 1986 May;49(5):1041-6. doi: 10.1016/S0006-3495(86)83732-8.
6
Acetylcholine receptor: channel-opening kinetics evaluated by rapid chemical kinetic and single-channel current measurements.乙酰胆碱受体：通过快速化学动力学和单通道电流测量评估的通道开放动力学
Biophys J. 1987 Nov;52(5):873-83. doi: 10.1016/S0006-3495(87)83281-2.
7
Single cholinergic receptor channel currents in cultured human muscle.培养的人体肌肉中的单胆碱能受体通道电流
J Neurosci. 1982 Oct;2(10):1465-73. doi: 10.1523/JNEUROSCI.02-10-01465.1982.
8
Ion flow gating by the acetylcholine system: kinetics of isolated receptor and esterase and of receptor-mediated ion flux.乙酰胆碱系统对离子流的门控作用：分离的受体与酯酶以及受体介导的离子通量的动力学
J Physiol (Paris). 1981 May;77(9):1061-72.
9
Kinetic differences between embryonic- and adult-type acetylcholine receptors in rat myotubes.大鼠肌管中胚胎型和成体型乙酰胆碱受体之间的动力学差异。
J Physiol. 1988 Feb;396:267-96. doi: 10.1113/jphysiol.1988.sp016962.
10
Acetylcholine receptor kinetics at slow fiber neuromuscular junctions.慢肌纤维神经肌肉接头处的乙酰胆碱受体动力学
Fed Proc. 1981 Sep;40(11):2614-7.
引用本文的文献
1
Regulation of NMDAR activation efficiency by environmental factors and subunit composition.环境因素和亚基组成对 NMDAR 激活效率的调节。
J Gen Physiol. 2025 Jan 6;157(1). doi: 10.1085/jgp.202413637. Epub 2024 Nov 22.
2
Exploring the Activation Process of the Glycine Receptor.探索甘氨酸受体的激活过程。
J Am Chem Soc. 2024 Sep 25;146(38):26297-26312. doi: 10.1021/jacs.4c08489. Epub 2024 Sep 16.
3
α Proline 277 Residues Regulate GABAR Gating through M2-M3 Loop Interaction in the Interface Region.α 脯氨酸 277 残基通过 GABAAR 界面区 M2-M3 环相互作用调节门控。
ACS Chem Neurosci. 2022 Nov 2;13(21):3044-3056. doi: 10.1021/acschemneuro.2c00401. Epub 2022 Oct 11.
4
Aggregated Markov Processes and Channel Gating Kinetics.聚合马尔可夫过程与通道门控动力学
J Res Natl Bur Stand (1977). 1985 Nov-Dec;90(6):517-520. doi: 10.6028/jres.090.053.
5
Sleep-related hypermotor epilepsy associated mutations uncover important kinetic roles of α4β2- nicotinic acetylcholine receptor intracellular structures.与睡眠相关的运动过度性癫痫相关突变揭示了α4β2-烟碱型乙酰胆碱受体细胞内结构的重要动力学作用。
PLoS One. 2021 Mar 3;16(3):e0247825. doi: 10.1371/journal.pone.0247825. eCollection 2021.
6
α Subunit Histidine 55 at the Interface between Extracellular and Transmembrane Domains Affects Preactivation and Desensitization of the GABA Receptor.α 亚单位组氨酸 55 位于细胞外和跨膜结构域的界面处，影响 GABA 受体的预激活和脱敏。
ACS Chem Neurosci. 2021 Feb 3;12(3):562-572. doi: 10.1021/acschemneuro.0c00781. Epub 2021 Jan 20.
7
NMDA receptor channel gating control by the pre-M1 helix.M1 前肽螺旋控制 NMDA 受体通道门控。
J Gen Physiol. 2020 Apr 6;152(4). doi: 10.1085/jgp.201912362.
8
Distinctive single-channel properties of α4β2-nicotinic acetylcholine receptor isoforms.α4β2 型烟碱型乙酰胆碱受体亚型的独特单通道特性。
PLoS One. 2019 Mar 7;14(3):e0213143. doi: 10.1371/journal.pone.0213143. eCollection 2019.
9
Isoform-specific mechanisms of α3β4*-nicotinic acetylcholine receptor modulation by the prototoxin lynx1.原毒素lynx1对α3β4* - 烟碱型乙酰胆碱受体调节的亚型特异性机制
FASEB J. 2017 Apr;31(4):1398-1420. doi: 10.1096/fj.201600733R. Epub 2017 Jan 18.
10
Catalyst-like modulation of transition states for CFTR channel opening and closing: new stimulation strategy exploits nonequilibrium gating.CFTR 通道开启和关闭的过渡态的类催化剂调节：利用非平衡门控的新刺激策略。
J Gen Physiol. 2014 Feb;143(2):269-87. doi: 10.1085/jgp.201311089. Epub 2014 Jan 13.
本文引用的文献
1
Acetylcholine receptor kinetics. A description from single-channel currents at snake neuromuscular junctions.乙酰胆碱受体动力学。来自蛇神经肌肉接头单通道电流的描述。
Biophys J. 1982 Sep;39(3):253-61. doi: 10.1016/S0006-3495(82)84515-3.
2
Single cholinergic receptor channel currents in cultured human muscle.培养的人体肌肉中的单胆碱能受体通道电流
J Neurosci. 1982 Oct;2(10):1465-73. doi: 10.1523/JNEUROSCI.02-10-01465.1982.
3
Single channel currents activated by gamma-aminobutyric acid, muscimol, and (-)-pentobarbital in cultured mouse spinal neurons.培养的小鼠脊髓神经元中由γ-氨基丁酸、蝇蕈醇和(-)-戊巴比妥激活的单通道电流。
J Neurosci. 1982 Jul;2(7):889-94. doi: 10.1523/JNEUROSCI.02-07-00889.1982.
4
Rapid kinetics of single glutamate-receptor channels.单个谷氨酸受体通道的快速动力学
Nature. 1982 Feb 4;295(5848):410-2. doi: 10.1038/295410a0.
5
Fluctuations in the microsecond time range of the current through single acetylcholine receptor ion channels.通过单个乙酰胆碱受体离子通道的电流在微秒时间范围内的波动。
Nature. 1981 Dec 3;294(5840):464-6. doi: 10.1038/294464a0.
6
Multiple conductance states of single acetylcholine receptor channels in embryonic muscle cells.胚胎肌肉细胞中单个乙酰胆碱受体通道的多种电导状态
Nature. 1981 Dec 3;294(5840):462-4. doi: 10.1038/294462a0.
7
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.用于从细胞和无细胞膜片进行高分辨率电流记录的改进膜片钳技术。
Pflugers Arch. 1981 Aug;391(2):85-100. doi: 10.1007/BF00656997.
8
Closure of membrane channels gated by glutamate receptors may be a two-step process.由谷氨酸受体门控的膜通道的关闭可能是一个两步过程。
Nature. 1982 Feb 18;295(5850):599-603. doi: 10.1038/295599a0.
9
On the stochastic properties of single ion channels.关于单离子通道的随机特性。
Proc R Soc Lond B Biol Sci. 1981 Mar 6;211(1183):205-35. doi: 10.1098/rspb.1981.0003.
10
An analysis of the dose-response curve at voltage-clamped frog-endplates.对电压钳制的青蛙终板处剂量反应曲线的分析。
Pflugers Arch. 1975 Oct 28;360(2):145-53. doi: 10.1007/BF00580537.
Zotero 插件