Michiels L, Maisin J R, Pedersen F S, Merregaert J
Int J Cancer. 1984 Apr 15;33(4):511-7. doi: 10.1002/ijc.2910330415.
The FBR murine osteosarcoma virus complex, isolated from a radiation-induced osteosarcoma of an X/Gf mouse causes the rapid appearance of osteosarcomas in newborn mice and transforms fibroblasts in vitro. The two components of the FBR-viral complex have been isolated separately in tissue culture: FBR-MuLV by end-point dilution and FBR-MuSV by the establishment of mouse [FBR-NP 117 (NIH 3T3)] and rat non-producer cell lines [FBR-NP415 (REF)]. The host range and RNase Tl fingerprint analysis of FBR-MuLV demonstrated a pattern closely related to, but distinguishable from, Akv-MuLV. Transformed cells from both mice and rats contain a rescuable FBR-MuSV genome. These pseudotypes produce foci in tissue culture and induce osteosarcomas in susceptible mouse strains. An FBR-MuSV (FBR-MuLV) cDNA probe detects a 5.2 kb HindIII and a 9.5 kb EcoRI FBR-MuSV-specific fragment in FBR-MuSV-transformed non-producer rat cells. The same fragments hybridized with a fos specific probe, demonstrating that FBR-provirus contains a c-fos-derived onc-gene.
从一只X/Gf小鼠的辐射诱导骨肉瘤中分离出的FBR鼠骨肉瘤病毒复合体，可使新生小鼠迅速出现骨肉瘤，并在体外转化成纤维细胞。FBR病毒复合体的两个组分已在组织培养中分别分离出来：通过终点稀释法分离出FBR-MuLV，通过建立小鼠[FBR-NP 117（NIH 3T3）]和大鼠非生产细胞系[FBR-NP415（REF）]分离出FBR-MuSV。FBR-MuLV的宿主范围和核糖核酸酶Tl指纹分析显示出一种与Akv-MuLV密切相关但又可区分的模式。来自小鼠和大鼠的转化细胞都含有可拯救的FBR-MuSV基因组。这些假型在组织培养中产生病灶，并在易感小鼠品系中诱发骨肉瘤。一个FBR-MuSV（FBR-MuLV）cDNA探针在FBR-MuSV转化的非生产大鼠细胞中检测到一个5.2 kb的HindIII和一个9.5 kb的EcoRI FBR-MuSV特异性片段。相同的片段与一个fos特异性探针杂交，表明FBR前病毒含有一个源自c-fos的致癌基因。
