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由NAD焦磷酸化酶催化的核苷酸转移的立体化学过程。

The stereochemical course of nucleotidyl transfer catalysed by NAD pyrophosphorylase.

作者信息

Lowe G, Tansley G

出版信息

Eur J Biochem. 1983 Apr 15;132(1):117-20. doi: 10.1111/j.1432-1033.1983.tb07334.x.

DOI:10.1111/j.1432-1033.1983.tb07334.x
PMID:6301818
Abstract

NAD pyrophosphorylase catalyses nucleotidyl transfer from adenosine (R)-5'-[alpha-17O]triphosphate to nicotinamide mononucleotide with inversion of configuration at the alpha-P giving (S)-[17O]NAD+. The simplest interpretation of this observation is that the adenylyl group is transferred directly from ATP to the co-substrate by an 'in line' mechanism. It is also shown that snake venom phosphodiesterase hydrolyses NAD+ regio-specifically at the adenylyl terminus of the pyrophosphate bond.

摘要

NAD焦磷酸化酶催化从腺苷（R）-5'-[α-17O]三磷酸向烟酰胺单核苷酸的核苷酸转移，α-P处构型翻转，生成（S）-[17O]NAD+。对这一观察结果最简单的解释是，腺苷酰基通过“直线型”机制直接从ATP转移到共底物上。研究还表明，蛇毒磷酸二酯酶在焦磷酸键的腺苷酰末端区域特异性地水解NAD+。

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