人腺病毒2型、5型和3型针对BclI、ClaI、PvuI和SphI核酸内切酶的限制性图谱。

Restriction maps of human adenovirus types 2, 5 and 3 for BclI, ClaI, pvul and SphI endonucleases.

作者信息

D'Halluin J C, Milleville M, Boulanger P A

出版信息

Gene. 1983 Jan-Feb;21(1-2):165-9. doi: 10.1016/0378-1119(83)90158-0.

PMID:6301946

Abstract

The sites of cleavage by BclI, ClaI, PvuI and SphI in the DNA from adenovirus (Ad) serotypes 2, 5 and 3 have been located. Certain site coordinates were in accord with nucleotide sequences already published. A small difference in size between the PvuI-E fragments from Ad2 and Ad5 confirmed the existence of a deletion in the N-terminal moiety of Ad5 hexon gene, as previously implied by interserotypic recombinants (Boursnell and Mautner, Virology 112 (1981) 198-209) and more recently by amino acid sequencing (Von Bahr-Lindström et al., Virology 118 (1982) 352-362).

摘要

已确定腺病毒（Ad）2型、5型和3型DNA中BclI、ClaI、PvuI和SphI的切割位点。某些位点坐标与已发表的核苷酸序列一致。Ad2和Ad5的PvuI - E片段在大小上的微小差异证实了Ad5六邻体基因N端部分存在缺失，这正如之前血清型间重组体所暗示的（Boursnell和Mautner，《病毒学》112卷（1981年）198 - 209页），以及最近氨基酸测序所表明的（Von Bahr - Lindström等人，《病毒学》118卷（1982年）352 - 362页）。