Hassell J A, Weber J
J Virol. 1978 Dec;28(3):671-8. doi: 10.1128/JVI.28.3.671-678.1978.
Temperature-sensitive mutations of human adenoviruses can be physically located on the viral genome by determining the DNA structures of recombinants formed in genetic crosses between different members of the same subgroup. We have analyzed the DNA structures of many interserotypic recombinants formed in crosses between temperature-sensitive (ts) mutants of adenovirus type 2 and adenovirus type 5 with the restriction endonucleases BamHI, EcoRI, HindIII, and and Sma I. In this way, we have mapped the physical coordinates of adenovirus type 2 (Ad2) ts1, Ad2 ts3, Ad2 ts4, and Ad2 ts48, and refined the mapping of Ad5 ts1.
通过确定同一亚组不同成员之间遗传杂交中形成的重组体的DNA结构，可以在病毒基因组上实际定位人腺病毒的温度敏感突变。我们用限制性内切酶BamHI、EcoRI、HindIII和Sma I分析了腺病毒2型和腺病毒5型的温度敏感（ts）突变体之间杂交形成的许多血清型间重组体的DNA结构。通过这种方式，我们绘制了腺病毒2型（Ad2）ts1、Ad2 ts3、Ad2 ts4和Ad2 ts48的物理坐标，并完善了Ad5 ts1的图谱绘制。
