Beck Y, Coetzee W F
Gene. 1983 Jan-Feb;21(1-2):25-31. doi: 10.1016/0378-1119(83)90144-0.
The gene for phenylalanine deaminase (PAD) of Proteus morganii strain 2815 has been isolated on a 6.3-kb HindIII restriction fragment and cloned within RP4-prime plasmids, pYB2321 and pYB2322, in both orientations. Expression of the cloned gene in Escherichia coli strains was comparable to that in P. morganii 2815. The hybrid plasmids mobilized the 2815 chromosome with trajectories in reverse directions from an origin between ser-2 and ade-1, suggesting the map location of the PAD gene.
摩根氏变形杆菌2815株的苯丙氨酸脱氨酶（PAD）基因已在一个6.3kb的HindIII限制性片段上分离出来，并以两种方向克隆到RP4-prime质粒pYB2321和pYB2322中。该克隆基因在大肠杆菌菌株中的表达与在摩根氏变形杆菌2815中的表达相当。这些杂交质粒从ser-2和ade-1之间的一个起点开始，以相反的方向移动2815染色体，这表明了PAD基因的图谱位置。
