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Cloning and nucleotide sequencing of phenylalanine dehydrogenase gene of Bacillus sphaericus.

作者信息

Okazaki N, Hibino Y, Asano Y, Ohmori M, Numao N, Kondo K

机构信息

Sagami Chemical Research Center, Kanagawa, Japan.

出版信息

Gene. 1988 Mar 31;63(2):337-41. doi: 10.1016/0378-1119(88)90537-9.

DOI:10.1016/0378-1119(88)90537-9
PMID:2838396
Abstract

The gene coding for phenylalanine dehydrogenase [PDH; L-phenylalanine: NAD+ oxidoreductase (deaminating); EC 1.4.1.-] from Bacillus sphaericus SCRC-79a was cloned onto plasmid pUC9, and the nucleotide sequence of the 2-kb DNA region of the insert was determined. A 1143-bp open reading frame consisting of 381 codons was identified as a pdh gene coding for PDH.

摘要

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