Tucker S D, Gopalakrishnan A S, Bollinger R, Dowhan W, Murgola E J
J Bacteriol. 1982 Nov;152(2):773-9. doi: 10.1128/jb.152.2.773-779.1982.
By the use of [5'-32P]tRNA3Gly from Escherichia coli as a hybridization probe, glyW was located on cloned fragments of the uvrC pgsA region of the bacterial chromosome. After determination of the sites of action of several restriction enzymes, glyW was found to be within approximately 300 base pairs of pgsA. The order of genes in this region is uvrC, pgsA, glyW, flaI. Comparison of the order of determined restriction sites with the sites predicted from the nucleotide sequence of tRNA3Gly indicates that the direction of transcription of glyW is counterclockwise on the circular E. coli map.
通过使用来自大肠杆菌的[5'-32P]tRNA3Gly作为杂交探针，glyW基因被定位在细菌染色体uvrC pgsA区域的克隆片段上。在确定了几种限制酶的作用位点后，发现glyW基因位于pgsA基因大约300个碱基对范围内。该区域的基因顺序为uvrC、pgsA、glyW、flaI。将确定的限制酶切位点顺序与根据tRNA3Gly核苷酸序列预测的位点进行比较，结果表明在环状大肠杆菌图谱上，glyW基因的转录方向是逆时针的。
