从肝细胞癌组织DNA克隆的乙肝序列的组织与表达

Organization and expression of hepatitis B sequences cloned from hepatocellular carcinoma tissue DNA.

作者信息

Dejean A, Carloni G, Bréchot C, Tiollais P, Wain-Hobson S

出版信息

J Cell Biochem. 1982;20(3):293-301. doi: 10.1002/jcb.240200309.

DOI:10.1002/jcb.240200309

Abstract

We have constructed a phage lambda library of liver DNA fragments from West African patient who died of liver failure due to advanced hepatocellular carcinoma. Four hepatitis B virus (HBV) DNA-carrying recombinants have been isolated, one clone (lambda IA22) being analyzed in greatest detail. It contains approximately 3.8 kb of HBV DNA without detectable deletions or rearrangements. One site of integration lies close to the nick in free viral DNA. The restriction map of the HBV sequences is close to those published for the ay subtype. Coconvection of mouse Ltk- cells with lambda IA22 and cloned thymidine kinase gene results in the expression of gene S and the excretion of hepatitis B surface antigen (HBsAg) particles into the culture supernatant.

摘要

我们构建了一个来自西非因晚期肝细胞癌导致肝功能衰竭死亡患者的肝脏DNA片段的λ噬菌体文库。已分离出四个携带乙型肝炎病毒（HBV）DNA的重组体，其中一个克隆（λIA22）得到了最详细的分析。它包含约3.8 kb的HBV DNA，未检测到缺失或重排。一个整合位点靠近游离病毒DNA中的切口。HBV序列的限制性图谱与已发表的ay亚型图谱相近。将小鼠Ltk-细胞与λIA22和克隆的胸苷激酶基因共转染，导致基因S表达并将乙型肝炎表面抗原（HBsAg）颗粒分泌到培养上清中。