Carloni G, Delfini C, Colloca S, Alfani E, Taliani G, De Bac C
Arch Virol. 1986;87(1-2):97-105. doi: 10.1007/BF01310546.
The presence of Hepatitis B virus (HBV) DNA in sera of patients with HBV related diseases is considered a reliable marker of virus active replication. In this paper HBV DNA was assayed by the molecular hybridization method with a 32P labeled nick translated HBV probe. The assay was positive in sera of 21 out of 22 HBeAg-positive and 4 out of 8 HBeAg-negative asymptomatic HBsAg carriers. 15 HBsAg-negative sera obtained from healthy donors showed no HBV DNA. Almost 80 per cent of HBeAg and HBV DNA positive sera revealed a DNA-polymerase activity. In order to determine the infectivity of HBsAg carriers, it appears that, whenever possible, the HBV DNA spot hybridization should be performed in conjunction with the DNA-polymerase, HBsAg and HBeAg tests.
乙型肝炎病毒（HBV）相关疾病患者血清中存在HBV DNA被认为是病毒活跃复制的可靠标志物。本文采用分子杂交法，用32P标记的缺口平移HBV探针检测HBV DNA。在22例HBeAg阳性无症状HBsAg携带者中，21例血清检测呈阳性；在8例HBeAg阴性无症状HBsAg携带者中，4例血清检测呈阳性。从健康献血者获得的15份HBsAg阴性血清未显示HBV DNA。几乎80%的HBeAg和HBV DNA阳性血清显示出DNA聚合酶活性。为了确定HBsAg携带者的传染性，似乎在可能的情况下，应将HBV DNA斑点杂交与DNA聚合酶、HBsAg和HBeAg检测结合进行。
