Arens M Q, Swierkosz E M
J Clin Microbiol. 1983 Mar;17(3):548-51. doi: 10.1128/jcm.17.3.548-551.1983.
We have developed a simplified method for unambiguously typing herpes simplex virus. The method depends on the production of cell-associated virus at 34 degrees C and subsequently, on the separation of cellular DNA and viral DNA by Dounce homogenization and the removal of nuclei by centrifugation. Viral nucleic acid was prepared from the cytoplasmic fraction and analyzed after restriction endonuclease cleavage. The method obviates the use of radioactive isotopes, and the viral DNA is effectively free of interfering cellular DNA.
我们已经开发出一种用于明确鉴定单纯疱疹病毒类型的简化方法。该方法依赖于在34摄氏度下产生细胞相关病毒,随后通过玻璃匀浆器匀浆分离细胞DNA和病毒DNA,并通过离心去除细胞核。从细胞质部分制备病毒核酸,并在限制性内切酶切割后进行分析。该方法无需使用放射性同位素,并且病毒DNA有效地不含干扰性的细胞DNA。